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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-422 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-422, RRID:AB_2102711
- Product name
- Rabbit Polyclonal FKBP15 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FKBP15 Antibody [NB100-422] - Detection of Human and Mouse FKPB-135 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FKPB-135 antibody NB100-422 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FKBP15 Antibody [NB100-422] - Detection of human FKBP-135 in Western Blot and immunoprecipitation, using NB100-422. Samples: HeLa nuclear extracts. Exposure times of (A) 25 minutes and (B) 10 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: FKBP15 Antibody [NB100-422] - Detection of human FKBP15 (FKPB-135) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FKBP15 antibody NB100-422 (lot 2) used for IP at 6 ug per reaction. FKBP15 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-FKBP15 antibody NB100-423. For blotting immunoprecipitated FKBP15, NB100-422 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
