MAB19591

antibody from Novus Biologicals

Targeting: IL12RB2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB19591

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-12 R beta 2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-12 R beta 2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-12 R beta 1 or recombinant mouse IL-12 R beta 1 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human IL-12 R beta 2 by Western Blot. Western blot shows lysates of human CD4⁺ in vitro differentiated Th1 cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human IL-12 R beta 2 Monoclonal Antibody (Catalog # MAB19591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-12 R beta 2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.