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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- FAB1959C-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-12 R beta 2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse IL-12 R beta 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-1 R beta 1 is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of IL-12 R beta 2 in Human TH1 cells by Flow Cytometry. Human CD4+ peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 ug/mL plate-bound Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100), 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL), and 10 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 5 days to induce Th1 cell development, followed by restimulation with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 2 to 3 hours, were stained with Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and Mouse Anti-Human/Mouse IL-12 R beta 2 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1959C). Quadrant markers were set based on control antibody staining using Mouse IgG1 PerCP Isotype Control (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins.
