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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB1959P-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse IL-12 R beta 2 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse IL-12 R beta 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-1 R beta 1 is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
Q99665- Isotype
- IgG
- Antibody clone number
- 305719
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references POSH Regulates CD4+ T Cell Differentiation and Survival.
Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
Cunningham CA, Cardwell LN, Guan Y, Teixeiro E, Daniels MA
Journal of immunology (Baltimore, Md. : 1950) 2016 May 15;196(10):4003-13
Journal of immunology (Baltimore, Md. : 1950) 2016 May 15;196(10):4003-13
Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
Freeman BE, Meyer C, Slifka MK
Journal of virology 2014 Jul;88(13):7474-84
Journal of virology 2014 Jul;88(13):7474-84
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-12 R beta 2 in Human TH1 cells by Flow Cytometry. Human CD4+ peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 ug/mL plate-bound Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100), 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL), and 10 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 5 days to induce Th1 cell development, followed by restimulation with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 2 to 3 hours, were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and Mouse Anti-Human/Mouse IL-12 R beta 2 PE-conjugated Monoclonal Antibody (Catalog # FAB1959P). Quadrant markers were set based on staining using Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
