F54552

antibody from NSJ Bioreagents

Targeting: IL12RB2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54552

Provider

NSJ Bioreagents

Product name

IL12RB2 Antibody

Antibody type

Polyclonal

Description

This highly specific IL12RB2 antibody is suitable for use in Immunohistochemistry/Western blot/Flow cytometry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the IL12RB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human MDA-MB-435 cell lysate with IL12RB2 antibody. Predicted molecular weight ~97 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) ThP-1, 2) A431, 3) Jurkat and 4) MOLT4 cell lysate with IL12RB2 antibody. Predicted molecular weight ~97 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil tissue with IL12RB2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human A431 cells with IL12RB2 antibody; Blue=isotype control, Green= IL12RB2 antibody.