Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00010181-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010181-M01, RRID:AB_547629
- Product name
- Mouse Monoclonal RBM5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RBM5 - RNA binding motif protein 5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM5 Antibody (2B6) [H00010181-M01] - Analysis of RBM5 over-expressed 293 cell line, cotransfected with RBM5 Validated Chimera RNAi ( Cat # H00010181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM5 monoclonal antibody (M01), clone 2B6 (Cat # H00010181-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM5 Antibody (2B6) [H00010181-M01] - Analysis of RBM5 expression in transfected 293T cell line by RBM5 monoclonal antibody (M01), clone 2B6.Lane 1: RBM5 transfected lysate(61.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RBM5 Antibody (2B6) [H00010181-M01] - Analysis of monoclonal antibody to RBM5 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RBM5 Antibody (2B6) [H00010181-M01] - Analysis of monoclonal antibody to RBM5 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.