H00010181-M01

antibody from Invitrogen Antibodies

Targeting: RBM5 H37, LUCA-15, LUCA15

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010181-M01

Provider

Invitrogen Antibodies

Product name

RBM5 Monoclonal Antibody (2B6)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: GYHSDGDYGE HDYRHDISDE RESKTIMLRG LPITITESDI REMMESFEGP QPADVRLMKR KTGVSRGFAF VEFYHLQDAT SWMEANQKKL VIQGKHIAMH YSNPRPKFED

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2B6

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBM5 over-expressed 293 cell line, cotransfected with RBM5 Validated Chimera RNAi (Cat # H00010181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM5 monoclonal antibody (M01), clone 2B6 (Cat # H00010181-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RBM5 expression in transfected 293T cell line by RBM5 monoclonal antibody (M01), clone 2B6.Lane 1: RBM5 transfected lysate(61.5 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBM5 over-expressed 293 cell line, cotransfected with RBM5 Validated Chimera RNAi (Cat # H00010181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM5 monoclonal antibody (M01), clone 2B6 (Cat # H00010181-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RBM5 expression in transfected 293T cell line by RBM5 monoclonal antibody (M01), clone 2B6.Lane 1: RBM5 transfected lysate(61.5 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to RBM5 on HeLa cell. Antibody concentration is 10 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to RBM5 on formalin-fixed paraffin-embedded human lung. Antibody concentration is 3 µg/mL.