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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-99683 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PEG3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total PEG3.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Padi2/3 Deficiency Alters the Epigenomic Landscape and Causes Premature Differentiation of Mouse Trophoblast Stem Cells.
Ballasy NN, Bering EA, Kokorudz C, Radford BN, Zhao X, Dean W, Hemberger M
Cells 2022 Aug 9;11(16)
Cells 2022 Aug 9;11(16)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PEG3 in A549 cell lysate. Samples were incubated with PEG3 polyclonal antibody (Product # PA5-99683).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PEG3 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and PEG3 polyclonal antibody (Product # PA5-99683) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded PEG3 in rat lung tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with PEG3 polyclonal antibody (Product # PA5-99683) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 CGI hypomethylation licenses trophoblast differentiation-promoting genes for precocious up-regulation. ( A ) Expression dynamics of trophoblast differentiation-relevant genes that are associated with hypomethylated CGIs in Padi2/3 DKO cells across a TSC differentiation time course. ( B ) Immunofluorescence staining for PEG3 indicates its up-regulation in TSCs after 3 days of differentiation. ( C ) RT-qPCR analysis showing the up-regulation of genes associated with hypomethylated CGIs ( Gata3_202 , 9230102O04Rik , Hand1 ) and TGC differentiation markers ( Prl2c2 , Prl3d1 ) in Padi2/3 DKO TSCs under stem cell conditions. Data are expressed relative to WT TSCs and are displayed as the mean +/- S.E.M. Statistical analysis by unpaired Student's t -test (* p < 0.05; ** p < 0.01; n = 4 independent clones per genotype). ( D ) Immunofluorescence staining for PEG3 showing stronger expression in the enlarged TGCs (arrow) that are evident in Padid2/3 DKO TSCs under stem cell conditions. ( E ) Immunostaining for FLAG-tagged PADI3 in TSCs revealing unequivocally nuclear localization (arrows) in TSCs. ( F ) Model depicting the role of PADI2/3 in TSCs. Both PADIs act synergistically to prevent precocious TGC differentiation by maintaining DNA methylation at CGIs that are associated with key trophoblast genes, preventing their premature up-regulation. This inhibitory effect towards TGC formation allows for the sufficient differentiation of SynT cells.
