Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-48285 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM65 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRIM65 in NCI-H460 cell line lysates (35 µg/lane). Lysates were probed with a TRIM65 Antibody (Center) (Product # PA5-48285).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of TRIM65 in NCI-H460 cells. Cells were stained with a TRIM65 Antibody (Center) (Product # PA5-48285) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TRIM65 in formalin fixed and paraffin embedded human prostate carcinoma tissue. Samples were probed with a TRIM65 Antibody (Center) (Product # PA5-48285) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of TRIM65 in NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). Samples were probed with a TRIM65 Antibody (Center) (Product # PA5-48285). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.