Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB7900 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Calponin 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Calponin 1 in ELISAs. Detects human, mouse and rat Calponin 1 in Western Blots. In direct ELISAs, no cross-reactivity with recombinant human Calponin 3 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P51911
- Isotype
- IgG
- Antibody clone number
- 836701
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Calponin 1 by Western Blot. Western blot shows lysates of MDA-MB-231 human breast cancer cell line, MCF-7 human breast cancer cell line, NMuMG mouse mammary gland epithelial cell line, and A7r5 rat thoracic aortic smooth muscle cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Calponin 1 Monoclonal Antibody (Catalog # MAB7900) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calponin 1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Calponin 1 in MDA-MD-231 Human Cell Line. Calponin 1 was detected in immersion fixed MDA-MD-231 human breast cancer cell line using Mouse Anti-Human Calponin 1 Monoclonal Antibody (Catalog # MAB7900) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Calponin 1 in Human Small Intestine. Calponin 1 was detected in immersion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human Calponin 1 Monoclonal Antibody (Catalog # MAB7900) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.