V7248

antibody from NSJ Bioreagents

Targeting: CNN1 Sm-Calp, SMCC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V7248

Provider

NSJ Bioreagents

Product name

Recombinant Calponin Antibody / Rabbit Monoclonal

Antibody type

Monoclonal

Description

This highly specific Recombinant Calponin antibody is suitable for use in Flow cytometry/Immunofluorescence/Immunohistochemistry/Western blot applications with human and rat samples.

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

CNN1/1408R

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the recombinant Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human colon tissue lysate with recombinant Calponin antibody. Predicted molecular weight ~34 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of permeabilized human K562 cells with recombinant Calponin antibody (green, clone CNN1/1408R) and Reddot nuclear stain (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat uterus stained with recombinant Calponin antibody (clone CNN1/1408R). HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat uterus stained with recombinant Calponin antibody (clone CNN1/1408R). Required HIER: steam sections in 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of PFA-fixed human K562 cells with recombinant Calponin antibody (clone CNN1/1408R); Red=isotype control, Blue= recombinant Calponin antibody.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free recombinant Calponin antibody (clone CNN1/1408R) as confirmation of integrity and purity.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free recombinant Calponin antibody (clone CNN1/1408R) as confirmation of integrity and purity.