Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- MA5-32061 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calponin 1 Recombinant Rabbit Monoclonal Antibody (SI67-01)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SI67-01
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calponin 1 in Hela cell lysate using a Calponin 1 Monoclonal antibody (Product # MA5-32061) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti- Calponin 1 Recombinant Rabbit Monoclonal Antibody (SI67-01) (Product # MA5-32061) and a 33 kDa band corresponding to CNN1 was observed across all the tissues tested with low expression in Mouse Adipose. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Adipose (Lane 2), and Mouse Skeletal Muscle (Lane 3) were electrophoresed Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Calponin 1 in HepG2 cells using a Calponin 1 Monoclonal antibody (Product # MA5-32061) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calponin 1 of paraffin-embedded Human breast tissue using a Calponin-1 Monoclonal antibody (Product #MA5-32061). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calponin 1 of paraffin-embedded Mouse bladder tissue using a Calponin-1 Monoclonal antibody (Product #MA5-32061). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calponin 1 of paraffin-embedded Mouse stomach tissue using a Calponin-1 Monoclonal antibody (Product #MA5-32061). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calponin 1 of paraffin-embedded Mouse heart tissue using a Calponin-1 Monoclonal antibody (Product #MA5-32061). Counter stained with hematoxylin.