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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- Ab092561 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Calponin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human Calponin 1 Antibody, Polyclonal, could be used for WB, IHC and so on.ApplicationWB: 1/500-1/1000IHC: 1/50-1/100Protein FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
- Reactivity
- Human, Mouse, Rat, Canine, Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 250-297- Vial size
- 100μl,10μl,1ml,50μl
- Concentration
- 0,4 mg/ml
- Storage
- Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Calponin 1 Antibody (Ab092561) - Western Blot All lanes: Calponin 1 Antibody (Ab092561) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 33 kDa Observed band size: 32, 34 kDa Exposure time: 22.5 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Calponin 1 Antibody (Ab092561) - Western Blot All lanes: Calponin 1 Antibody (Ab092561) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 33 kDa Observed band size: 32, 34 kDa Exposure time: 20.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Calponin 1 Antibody (Ab092561) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast using Calponin 1 Antibody (Ab092561). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
