21404-1-AP

antibody from Proteintech Group

Targeting: MYH11 SMHC, SMMHC

Provider product page for 21404-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [65]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [4]

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Product number: 21404-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21404-1-AP, RRID:AB_10732819
Product name: SMMHC antibody
Antibody type: Polyclonal
Description: SMMHC antibody (Cat. #21404-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MYH11 protein structure - 21404-1-AP shown in red.

Submitted references RhoA accelerates atherosclerosis progression by interacting with Hspa5.

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Store-operated Ca(2+) entry contributes to the ASM phenotype transition in asthma.

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Ma X, Xu J, Sun H, Liu J, Xia S, Zhang H, Cui C, Song C
Experimental biology and medicine (Maywood, N.J.) 2025;250:10527

Single-Cell RNA Sequencing Reveals the Heterogeneity in Differentiation Trajectory and Tumor Microenvironment Leading to More Aggressive Phenotypes of Papillary Thyroid Cancer in Children and Young Adult Patients.

Guo K, Yang L, Huang Z, Qian K, Shi Y, Fei M, Feng Y, Chen Z, Qiu M, Tang L, Li A, Lv Z, Liu J, Chen Z, Dong K, Wang Z
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Vasostatin-2 attenuates injury-induced neointimal hyperplasia through the ACE2/MasR/PPARγ/NR1D1/Gas1 axis.

Chen Q, Liu J, Madonna R, Li F, Chen S, Li L, Wu X, Maimati Y, Ding F, Wang X, Shen Y, Zhang R, Shen W, Dai Y, Lu L, De Caterina R
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Dehydrocorydaline maintains the vascular smooth muscle cell contractile phenotype by upregulating Spta1.

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Sulfur Dioxide Alleviates Aortic Dissection Through Inhibiting Vascular Smooth Muscle Cell Phenotype Switch, Migration, and Proliferation via miR-184-3p/Cyp26b1 Axis.

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MMP19 in vascular smooth muscle cells protects against thoracic aortic aneurysm and dissection via the MMP19/Aggrecan/Wnt/β-catenin axis.

Ma B, Zeng Q, Yang F, Yang H, Li W, Fu R, Cai Z, Zhu G, Shu C, Luo M, Zhou Z
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Smooth muscle cell Piezo1 is essential for phenotypic switch and neointimal hyperplasia.

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Differential aortic aneurysm formation provoked by chemogenetic oxidative stress.

Das AA, Waldeck-Weiermair M, Yadav S, Spyropoulos F, Pandey A, Dutta T, Covington TA, Michel T
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Relationship Between Vascular Smooth Muscle Cell Phenotype and Degeneration of Elastin in the Aortic Media in Patients With Acute Aortic Dissection.

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CJC open 2025 Jun;7(6):788-794

Smooth muscle-specific expression of hydroxyindole O-methyltransferase reduces arterial injury-induced intimal hyperplasia.

Jiang WC, Chen CH, Ho HH, Gung PY, Wu JY, Kuo CC, Wu KK, Yet SF
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Pericytes promote metastasis by regulating tumor local vascular tone and hemodynamics.

Li X, Yan S, Wu X, Miao Q, Zhang D, Mai W, Han S, Tang Z, Ye M, Zhang S, Wei JA, Pan J, Huang D, Qiu S, Zhao Z, Zhong X, Huang M, Qi M, Zhang J, Wang C, Xie J, Wang S, Luo OJ, Zhang D, Ye W, Chen M
Nature communications 2025 Aug 2;16(1):7115

Isovaleroylbinankadsurin A ameliorates atherosclerosis and restenosis by promoting LXRα signaling pathway and inhibiting TGF-β1 and FHL1 signaling pathways.

Shi Y, Liu L, Gong Y, Zhang C, Yang Y, Wang W, Qin L
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Activation of nuclear receptor pregnane-X-receptor protects against abdominal aortic aneurysm by inhibiting oxidative stress.

Shen Z, Wang J, Chen Y, Fang P, Yuan A, Chen AF, Yan X, Lyu Y, Pu J
Redox biology 2024 Nov;77:103397

Tryptophan 2,3-dioxygenase-positive matrix fibroblasts fuel breast cancer lung metastasis via kynurenine-mediated ferroptosis resistance of metastatic cells and T cell dysfunction.

Liu Y, Chen S, Wan X, Wang R, Luo H, Chang C, Dai P, Gan Y, Guo Y, Hou Y, Sun Y, Teng Y, Cui X, Liu M
Cancer communications (London, England) 2024 Nov;44(11):1261-1286

Mechanical memory based on chromatin and metabolism remodeling promotes proliferation and smooth muscle differentiation in mesenchymal stem cells.

Na J, Shi Q, Yang Z, Liu Y, Chen X, Wang Z, Zheng L, Fan Y
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Fibulin 1, targeted by microRNA-24-3p, promotes cell proliferation and migration in vascular smooth muscle cells, contributing to the development of atherosclerosis in APOE(-/-) mice.

Ji T, Yan D, Huang Y, Luo M, Zhang Y, Xu T, Gao S, Zhang L, Ruan L, Zhang C
Gene 2024 Mar 10;898:148129

Bergenin alleviates proliferative arterial diseases by modulating glucose metabolism in vascular smooth muscle cells.

Song Y, Deng M, Qiu Y, Cui Y, Zhang B, Xin J, Feng L, Mu X, Cui J, Li H, Sun Y, Yi W
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jul;129:155592

A proteome-wide screen identifies the calcium binding proteins, S100A8/S100A9, as clinically relevant therapeutic targets in aortic dissection.

Jiang H, Zhao Y, Su M, Sun L, Chen M, Zhang Z, Ilyas I, Wang Z, Little PJ, Wang L, Weng J, Ge J, Xu S
Pharmacological research 2024 Jan;199:107029

Chitosan inhibits vascular intimal hyperplasia via LINC01615/MIR-185-5p/PIK3R2 signaling pathway.

Yan Y, Wu Q, Li JH, Wei X, Xiao J, Yang L, Xie A, Zhang L, Mei WJ, Yang YJ, Zeng Y, Wen D, Deng LJ, Zheng LF
Gene 2024 Jan 20;892:147850

microRNA-18a-5p promotes vascular smooth muscle cell phenotypic switch by targeting Notch2 as therapeutic targets in vein grafts restenosis.

Zhan X, Zhong CM, Tang H, Xiao H, Guo Y, Zhang C, Qu C, Wang X, Huang C
European journal of pharmacology 2024 Dec 15;985:177097

Suramin inhibits phenotypic transformation of vascular smooth muscle cells and neointima hyperplasia by suppressing transforming growth factor beta receptor 1 /Smad2/3 pathway activation.

Xu Y, Ge L, Rui Y, Wang Y, Wang Z, Yang J, Shi Y, Dong Z, Zhang Y, Zhang R, Yang T, Lv L, Xiang F, Chen S, Song A, Li T, Liu M, Guo Y
European journal of pharmacology 2024 Apr 5;968:176422

Inhibition of tiRNA-Gly-GCC ameliorates neointimal formation via CBX3-mediated VSMCs phenotypic switching.

Rong Z, Li F, Zhang R, Niu S, Di X, Ni L, Liu C
Frontiers in cardiovascular medicine 2023;10:1030635

Musashi-2 binds with Fbxo6 to induce Rnaset2 ubiquitination and chemokine signaling pathway during vascular smooth muscle cell phenotypic switch in atherosclerosis.

Zhang T, Wu S, Xu R, Zhang S, Wang M, Li J
Cellular signalling 2023 Nov;111:110869

Morphological and Functional Colonic Defects Caused by a Mutated Thyroid Hormone Receptor α.

Kim M, Kruhlak M, Hoffmann V, Zerfas P, Bishop K, Doolittle WKL, Edmondson EF, Zhu YJ, Cheng SY
Thyroid : official journal of the American Thyroid Association 2023 Feb;33(2):239-250

Ant-Neointimal Formation Effects of SLC6A6 in Preventing Vascular Smooth Muscle Cell Proliferation and Migration via Wnt/β-Catenin Signaling.

Rong Z, Li F, Zhang R, Niu S, Di X, Ni L, Liu C
International journal of molecular sciences 2023 Feb 3;24(3)

Fate mapping RNA-sequencing reveal Malat1 regulates Sca1(+) progenitor cells to vascular smooth muscle cells transition in vascular remodeling.

Lyu L, Li Z, Wen Z, He Y, Wang X, Jiang L, Zhou X, Huang C, Wu Y, Chen T, Guo X
Cellular and molecular life sciences : CMLS 2023 Apr 6;80(5):118

Tanreqing Injection Regulates Cell Function of Hypoxia-Induced Human Pulmonary Artery Smooth Muscle Cells (HPASMCs) through TRPC1/CX3CL1 Signaling Pathway.

Xiang Y, Zheng F, Zhang Q, Zhang R, Pan H, Pang Z, Dai S, Zhang Y, Wu Y, Yao L, Su M, Lan L
Oxidative medicine and cellular longevity 2022;2022:3235102

Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA(+) interstitial cells are fibroblasts.

Clayton DR, Ruiz WG, Dalghi MG, Montalbetti N, Carattino MD, Apodaca G
American journal of physiology. Renal physiology 2022 Sep 1;323(3):F299-F321

SARS-CoV-2 hijacks macropinocytosis to facilitate its entry and promote viral spike-mediated cell-to-cell fusion.

Zhang YY, Liang R, Wang SJ, Ye ZW, Wang TY, Chen M, Liu J, Na L, Yang YL, Yang YB, Yuan S, Yin X, Cai XH, Tang YD
The Journal of biological chemistry 2022 Nov;298(11):102511

Generation and Comparative Analysis of an Itga8-CreER (T2) Mouse with Preferential Activity in Vascular Smooth Muscle Cells.

Warthi G, Faulkner JL, Doja J, Ghanam AR, Gao P, Yang AC, Slivano OJ, Barris CT, Kress TC, Zawieja SD, Griffin SH, Xie X, Ashworth A, Christie CK, Bryant WB, Kumar A, Davis MJ, Long X, Gan L, Belin de Chantemele EJ, Lyu Q, Miano JM
Nature cardiovascular research 2022 Nov;1(11):1084-1100

Circ_0021155 can participate in the phenotypic transformation of human vascular smooth muscle cells via the miR-4459/TRPM7 axis.

Lin J, Liu C, Xu J, Li S, Dai D, Zhang L, Yonghui P
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Cell contractility and focal adhesion kinase control circumferential arterial stiffness.

Roberts E, Xu T, Assoian RK
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Intraoperative Hypothermia Induces Vascular Dysfunction in the CA1 Region of Rat Hippocampus.

Li T, Xu G, Yi J, Huang Y
Brain sciences 2022 May 27;12(6)

MYPT1 reduction is a pathogenic factor of erectile dysfunction.

Zhao W, Sun J, Yao LY, Hang D, Li YQ, Chen CP, Zhou YW, Chen X, Tao T, Wei LS, Zheng YY, Ge X, Li CJ, Xin ZC, Pan Y, Wang XZ, He WQ, Zhang XN, Yao B, Zhu MS
Communications biology 2022 Jul 25;5(1):744

PVT1/miR-145-5p/HK2 modulates vascular smooth muscle cells phenotype switch via glycolysis: The new perspective on the spiral artery remodeling.

Wu M, Liu W, Huang H, Chen Z, Chen Y, Zhong Y, Jin Z, Liu X, Zou L
Placenta 2022 Dec;130:25-33

Angiogenic factor AGGF1 blocks neointimal formation after vascular injury via interaction with integrin α7 on vascular smooth muscle cells.

Yu Y, Li Y, Peng H, Song Q, Da X, Li H, He Z, Ren X, Xu C, Yao Y, Wang QK
The Journal of biological chemistry 2022 Apr;298(4):101759

S-Nitroso-L-Cysteine Ameliorated Pulmonary Hypertension in the MCT-Induced Rats through Anti-ROS and Anti-Inflammatory Pathways.

Wang M, Luo P, Shi W, Guo J, Huo S, Yan D, Peng L, Zhang C, Lv J, Lin L, Li S
Oxidative medicine and cellular longevity 2021;2021:6621232

MicroRNA‑199a‑5p regulates FOXC2 to control human vascular smooth muscle cell phenotypic switch.

Cao Y, Cao Z, Wang W, Jie X, Li L
Molecular medicine reports 2021 Sep;24(3)

TWIST1 induces phenotypic switching of vascular smooth muscle cells by downregulating p68 and microRNA-143/145.

Zhang J, Guo JR, Wu XL, Wang X, Zhu ZM, Wang Y, Gu X, Fan Y
FEBS open bio 2021 Mar;11(3):932-943

Modulation of Vascular Smooth Muscle Cell Phenotype by High Mobility Group AT-Hook 1.

Jung Y, Lee HS, Ha JM, Jin SY, Kum HJ, Vafaeinik F, Ha HK, Song SH, Kim CD, Bae SS
Journal of lipid and atherosclerosis 2021 Jan;10(1):99-110

Prostacyclin facilitates vascular smooth muscle cell phenotypic transformation via activating TP receptors when IP receptors are deficient.

Li Z, Luo W, Fang S, Chen X, Lin T, Zhou S, Zhang L, Yang W, Li Z, Ye J, Wang J, Liu P, Li Z
Acta physiologica (Oxford, England) 2021 Feb;231(2):e13555

Neonatal Streptococcus Pneumoniae pneumonia induces airway SMMHC expression through HMGB1/TLR4/ERK.

Li Y, Guo Z, Zhang G, Tian X, Li Q, Luo Z
Immunology letters 2021 Dec;240:149-158

Coronary blood vessels from distinct origins converge to equivalent states during mouse and human development.

Phansalkar R, Krieger J, Zhao M, Kolluru SS, Jones RC, Quake SR, Weissman I, Bernstein D, Winn VD, D'Amato G, Red-Horse K
eLife 2021 Dec 15;10

Senescent cells suppress innate smooth muscle cell repair functions in atherosclerosis.

Childs BG, Zhang C, Shuja F, Sturmlechner I, Trewartha S, Fierro Velasco R, Baker D, Li H, van Deursen JM
Nature aging 2021 Aug;1(8):698-714

Vascular Adhesion Protein-1 Determines the Cellular Properties of Endometrial Pericytes.

Gharanei S, Fishwick K, Peter Durairaj R, Jin T, Siamantouras E, Liu KK, Straube A, Lucas ES, Weston CJ, Rantakari P, Salmi M, Jalkanen S, Brosens JJ, Tan BK
Frontiers in cell and developmental biology 2020;8:621016

Bioinformatics Analysis Reveals MicroRNA-193a-3p Regulates ACTG2 to Control Phenotype Switch in Human Vascular Smooth Muscle Cells.

Wang W, Wang Y, Piao H, Li B, Zhu Z, Li D, Wang T, Liu K
Frontiers in genetics 2020;11:572707

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Yang GS, Zheng B, Qin Y, Zhou J, Yang Z, Zhang XH, Zhao HY, Yang HJ, Wen JK
Theranostics 2020;10(17):7787-7811

Long non‑coding RNA MALAT1 sponges miR‑124‑3p.1/KLF5 to promote pulmonary vascular remodeling and cell cycle progression of pulmonary artery hypertension.

Wang D, Xu H, Wu B, Jiang S, Pan H, Wang R, Chen J
International journal of molecular medicine 2019 Sep;44(3):871-884

Artemisinin attenuates the development of atherosclerotic lesions by the regulation of vascular smooth muscle cell phenotype switching.

Du H, Zhao Q, Zang H, Chang C, Li X
Life sciences 2019 Nov 15;237:116943

Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2.

Wang C, Xu W, An J, Liang M, Li Y, Zhang F, Tong Q, Huang K
Nature communications 2019 Mar 13;10(1):1203

Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction.

Iguchi N, Dönmez Mİ, Carrasco A Jr, Wilcox DT, Pineda RH, Malykhina AP, Cost NG
American journal of physiology. Renal physiology 2019 Jul 1;317(1):F197-F206

Autophagy is involved in the differentiation of epicardial progenitor cells into vascular smooth muscle cells in mice.

Liu Y, Liu B, Li Y, Li Y, Du J, Deng S, Jing X, She Q
Experimental cell research 2019 Feb 15;375(2):60-71

CTRP13 attenuates vascular calcification by regulating Runx2.

Li Y, Wang W, Chao Y, Zhang F, Wang C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Aug;33(8):9627-9637

Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ.

Zhang JR, Lu QB, Feng WB, Wang HP, Tang ZH, Cheng H, Du Q, Wang YB, Li KX, Sun HJ
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Jun;102:711-717

UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease.

Elia L, Kunderfranco P, Carullo P, Vacchiano M, Farina FM, Hall IF, Mantero S, Panico C, Papait R, Condorelli G, Quintavalle M
The Journal of clinical investigation 2018 Jun 1;128(6):2473-2486

CD137-CD137L interaction modulates neointima formation and the phenotype transformation of vascular smooth muscle cells via NFATc1 signaling.

Zhong W, Li B, Yang P, Chen R, Wang C, Wang Z, Shao C, Yuan W, Yan J
Molecular and cellular biochemistry 2018 Feb;439(1-2):65-74

The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling.

Jourd'heuil FL, Xu H, Reilly T, McKellar K, El Alaoui C, Steppich J, Liu YF, Zhao W, Ginnan R, Conti D, Lopez-Soler R, Asif A, Keller RK, Schwarz JJ, Thanh Thuy LT, Kawada N, Long X, Singer HA, Jourd'heuil D
Arteriosclerosis, thrombosis, and vascular biology 2017 Oct;37(10):1944-1955

Oral chromium picolinate impedes hyperglycemia-induced atherosclerosis and inhibits proatherogenic protein TSP-1 expression in STZ-induced type 1 diabetic ApoE(-/-) mice.

Ganguly R, Sahu S, Ohanyan V, Haney R, Chavez RJ, Shah S, Yalamanchili S, Raman P
Scientific reports 2017 Mar 27;7:45279

Targeting AGGF1 (angiogenic factor with G patch and FHA domains 1) for Blocking Neointimal Formation After Vascular Injury.

Yao Y, Hu Z, Ye J, Hu C, Song Q, Da X, Yu Y, Li H, Xu C, Chen Q, Wang QK
Journal of the American Heart Association 2017 Jun 25;6(6)

Regulated lysosomal exocytosis mediates cancer progression.

Machado E, White-Gilbertson S, van de Vlekkert D, Janke L, Moshiach S, Campos Y, Finkelstein D, Gomero E, Mosca R, Qiu X, Morton CL, Annunziata I, d'Azzo A
Science advances 2015 Dec;1(11):e1500603

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21404-1-AP(MYH11 antibody) at dilution of 1:3000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The MYH11 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human MYH11. This antibody recognizes human, mouse antigen. The MYH11 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 21404-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon using 21404-1-AP(MYH11 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human skeletal muscle using 21404-1-AP(MYH11 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human skeletal muscle using 21404-1-AP(MYH11 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue