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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Other assay [1]
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- Product number
- TA347251 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal MYH11 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal MYH11 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MYH11
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibodies against CBFb (lane 1) and MYH11 ( lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human MYH11. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ChIP assays were performed using ME-1 cells, the antibody against MYH11 and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Image shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
- Validation comment
- Assay
