NBP2-59221

antibody from Novus Biologicals

Targeting: MYH11 SMHC, SMMHC

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-59221

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Myosin heavy chain 11 Antibody

Antibody type

Polyclonal

Description

Whole Antiserum.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Myosin heavy chain 11 Antibody [NBP2-59221] - Nuclear extracts of ME-1 cells were analysed by Western blot using the antibodies against CBFb and MYH11 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: Myosin heavy chain 11 Antibody [NBP2-59221] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MYH11. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,900.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation: Myosin heavy chain 11 Antibody [NBP2-59221] - ChIP assays were performed using ME-1 cells, the antibody against MYH11 and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 uL of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.