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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00001491-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001491-M03, RRID:AB_538058
- Product name
- Mouse Monoclonal Cystathionase Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CTH - cystathionase (cystathionine gamma-lyase)
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Cystathionine-γ-lyase expression is associated with mitochondrial respiration during sepsis-induced acute kidney injury in swine with atherosclerosis.
Relationship between selenoprotein P and selenocysteine lyase: Insights into selenium metabolism.
Increased ophthalmic acid production is supported by amino acid catabolism under fasting conditions in mice.
The viability of primary hepatocytes is maintained under a low cysteine-glutathione redox state with a marked elevation in ophthalmic acid production.
Ascorbic acid prevents acetaminophen-induced hepatotoxicity in mice by ameliorating glutathione recovery and autophagy.
Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry.
Hydrogen sulfide impairs glucose utilization and increases gluconeogenesis in hepatocytes.
cGMP-dependent protein kinase contributes to hydrogen sulfide-stimulated vasorelaxation.
An investigation of the mechanisms of hydrogen sulfide-induced vasorelaxation in rat middle cerebral arteries.
Characterization of hydrogen sulfide and its synthases, cystathionine β-synthase and cystathionine γ-lyase, in human prostatic tissue and cells.
Hydrogen sulfide mediates the anti-survival effect of sulforaphane on human prostate cancer cells.
Glucocorticoids suppress cystathionine gamma-lyase expression and H2S production in lipopolysaccharide-treated macrophages.
H2S contributes to the hepatic arterial buffer response and mediates vasorelaxation of the hepatic artery via activation of K(ATP) channels.
Merz T, Wepler M, Nußbaum B, Vogt J, Calzia E, Wang R, Szabo C, Radermacher P, McCook O
Intensive care medicine experimental 2018 Oct 20;6(1):43
Intensive care medicine experimental 2018 Oct 20;6(1):43
Relationship between selenoprotein P and selenocysteine lyase: Insights into selenium metabolism.
Seale LA, Ha HY, Hashimoto AC, Berry MJ
Free radical biology & medicine 2018 Nov 1;127:182-189
Free radical biology & medicine 2018 Nov 1;127:182-189
Increased ophthalmic acid production is supported by amino acid catabolism under fasting conditions in mice.
Kobayashi S, Lee J, Takao T, Fujii J
Biochemical and biophysical research communications 2017 Sep 23;491(3):649-655
Biochemical and biophysical research communications 2017 Sep 23;491(3):649-655
The viability of primary hepatocytes is maintained under a low cysteine-glutathione redox state with a marked elevation in ophthalmic acid production.
Lee J, Kang ES, Kobayashi S, Homma T, Sato H, Seo HG, Fujii J
Experimental cell research 2017 Dec 1;361(1):178-191
Experimental cell research 2017 Dec 1;361(1):178-191
Ascorbic acid prevents acetaminophen-induced hepatotoxicity in mice by ameliorating glutathione recovery and autophagy.
Kurahashi T, Lee J, Nabeshima A, Homma T, Kang ES, Saito Y, Yamada S, Nakayama T, Yamada K, Miyata S, Fujii J
Archives of biochemistry and biophysics 2016 Aug 15;604:36-46
Archives of biochemistry and biophysics 2016 Aug 15;604:36-46
Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry.
Ohmura M, Hishiki T, Yamamoto T, Nakanishi T, Kubo A, Tsuchihashi K, Tamada M, Toue S, Kabe Y, Saya H, Suematsu M
Nitric oxide : biology and chemistry 2015 Apr 30;46:102-13
Nitric oxide : biology and chemistry 2015 Apr 30;46:102-13
Hydrogen sulfide impairs glucose utilization and increases gluconeogenesis in hepatocytes.
Zhang L, Yang G, Untereiner A, Ju Y, Wu L, Wang R
Endocrinology 2013 Jan;154(1):114-26
Endocrinology 2013 Jan;154(1):114-26
cGMP-dependent protein kinase contributes to hydrogen sulfide-stimulated vasorelaxation.
Bucci M, Papapetropoulos A, Vellecco V, Zhou Z, Zaid A, Giannogonas P, Cantalupo A, Dhayade S, Karalis KP, Wang R, Feil R, Cirino G
PloS one 2012;7(12):e53319
PloS one 2012;7(12):e53319
An investigation of the mechanisms of hydrogen sulfide-induced vasorelaxation in rat middle cerebral arteries.
Streeter E, Hart J, Badoer E
Naunyn-Schmiedeberg's archives of pharmacology 2012 Oct;385(10):991-1002
Naunyn-Schmiedeberg's archives of pharmacology 2012 Oct;385(10):991-1002
Characterization of hydrogen sulfide and its synthases, cystathionine β-synthase and cystathionine γ-lyase, in human prostatic tissue and cells.
Guo H, Gai JW, Wang Y, Jin HF, Du JB, Jin J
Urology 2012 Feb;79(2):483.e1-5
Urology 2012 Feb;79(2):483.e1-5
Hydrogen sulfide mediates the anti-survival effect of sulforaphane on human prostate cancer cells.
Pei Y, Wu B, Cao Q, Wu L, Yang G
Toxicology and applied pharmacology 2011 Dec 15;257(3):420-8
Toxicology and applied pharmacology 2011 Dec 15;257(3):420-8
Glucocorticoids suppress cystathionine gamma-lyase expression and H2S production in lipopolysaccharide-treated macrophages.
Zhu XY, Liu SJ, Liu YJ, Wang S, Ni X
Cellular and molecular life sciences : CMLS 2010 Apr;67(7):1119-32
Cellular and molecular life sciences : CMLS 2010 Apr;67(7):1119-32
H2S contributes to the hepatic arterial buffer response and mediates vasorelaxation of the hepatic artery via activation of K(ATP) channels.
Siebert N, Cantré D, Eipel C, Vollmar B
American journal of physiology. Gastrointestinal and liver physiology 2008 Dec;295(6):G1266-73
American journal of physiology. Gastrointestinal and liver physiology 2008 Dec;295(6):G1266-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cystathionase Antibody (S51) [H00001491-M03] - Analysis of CTH expression in transfected 293T cell line by CTH monoclonal antibody (M03), clone S51. Lane 1: CTH transfected lysatE (45 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cystathionase Antibody (S51) [H00001491-M03] - Analysis of CTH expression in K-562 (Cat # L009V1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cystathionase Antibody (S51) [H00001491-M03] - Analysis of CTH expression in mouse kidney.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Cystathionase Antibody (S51) [H00001491-M03] - Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cystathionase Antibody (S51) [H00001491-M03] - Analysis of monoclonal antibody to CTH on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml
