UM500081

antibody from Invitrogen Antibodies

Targeting: ZSCAN18 FLJ12895, ZNF447

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

UM500081

Provider

Invitrogen Antibodies

Product name

ZSCAN18 Monoclonal Antibody (UMAB114), UltraMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

UMAB114

Vial size

100 µL

Concentration

0.5-1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of cell lysates (35 µg) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZSCAN18mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human thyroid tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human endometrium tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human lymph node tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human Kidney tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ZSCAN18 mouse monoclonal antibody (UM500081). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.