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- References [42]
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- Product number
- 10670-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10670-1-AP, RRID:AB_2195809
- Product name
- SPHK1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SPHK1 antibody (Cat. #10670-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Assessment of the Effects of Sphingosine Kinase 1/Sphingosine-1-Phosphate on Microangiogenesis at Rat Myofascial Trigger Points Using Contrast-Enhanced Ultrasonography.
Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia.
SPHK1 potentiates colorectal cancer progression and metastasis via regulating autophagy mediated by TRAF6-induced ULK1 ubiquitination.
Sphingosine kinase 1-specific inhibitor PF543 reduces goblet cell metaplasia of bronchial epithelium in an acute asthma model.
Protocol for the isolation of tumor cell-derived extracellular vesicles followed by in vivo metastasis assessment in a murine ovarian cancer model.
SPHK1/S1P/S1PR pathway promotes the progression of peritoneal fibrosis by mesothelial-mesenchymal transition.
ATF3 regulates SPHK1 in cardiomyocyte injury via endoplasmic reticulum stress.
UCHL1 Regulates Radiation Lung Injury via Sphingosine Kinase-1.
YTHDF1 mitigates acute kidney injury via safeguarding m(6)A-methylated mRNAs in stress granules of renal tubules.
Sphingosine Kinases at the Intersection of Pro-Inflammatory LPS and Anti-Inflammatory Endocannabinoid Signaling in BV2 Mouse Microglia Cells.
Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer.
A novel co-target of ACY1 governing plasma membrane translocation of SphK1 contributes to inflammatory and neuropathic pain.
The preventive effect of mussel oil on gestational diabetes mellitus in pregnant mice fed by a high-fat and high-sucrose diet.
A comprehensive multiomics approach reveals that high levels of sphingolipids in cardiac cachexia adipose tissue are associated with inflammatory and fibrotic changes.
Establishment of a Rat Model of Liver Venous Deprivation: Simultaneous Portal and Hepatic Vein Ligation.
LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion.
Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2.
Geniposide inhibits SphK1 membrane targeting to restore macrophage polarization balance in collagen-induced arthritis mice.
Metabolomic Analysis Reveals that SPHK1 Promotes Oral Squamous Cell Carcinoma Progression through NF-κB Activation.
Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.
Berberine ameliorates erectile dysfunction in rats with streptozotocin-induced diabetes mellitus through the attenuation of apoptosis by inhibiting the SPHK1/S1P/S1PR2 and MAPK pathways.
Identifying the mechanisms and molecular targets of Hongjingtian injection on treatment of TGFβ1-induced HK-2 cells: coupling network pharmacology with experimental verification.
Proteomic-Based Approach Reveals the Involvement of Apolipoprotein A-I in Related Phenotypes of Autism Spectrum Disorder in the BTBR Mouse Model.
CeO(2) nanoparticles induce pulmonary fibrosis via activating S1P pathway as revealed by metabolomics.
Potential of a sphingosine 1-phosphate receptor antagonist and sphingosine kinase inhibitors as targets for multiple myeloma treatment.
Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture.
SphK2/S1P Promotes Metastasis of Triple-Negative Breast Cancer Through the PAK1/LIMK1/Cofilin1 Signaling Pathway.
SPHK1 contributes to cisplatin resistance in bladder cancer cells via the NONO/STAT3 axis.
Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.
Bone marrow mesenchymal stem cells-derived exosomes reduce Aβ deposition and improve cognitive function recovery in mice with Alzheimer's disease by activating sphingosine kinase/sphingosine-1-phosphate signaling pathway.
POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling.
Sphingosine kinase 1 knockout alleviates hepatic ischemia/reperfusion injury by attenuating inflammation and oxidative stress in mice.
Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.
Sphingosine kinase 1 promotes the metastasis of colorectal cancer by inducing the epithelial‑mesenchymal transition mediated by the FAK/AKT/MMPs axis.
Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.
Bidirectional effects of moxifloxacin on the pro‑inflammatory response in lipopolysaccharide‑stimulated mouse peritoneal macrophages.
SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells.
Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1).
ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.
Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1.
MiR-506 suppresses liver cancer angiogenesis through targeting sphingosine kinase 1 (SPHK1) mRNA.
Phosphorylation and action of the immunomodulator FTY720 inhibits vascular endothelial cell growth factor-induced vascular permeability.
Fang X, Yin Y, Lun H, Liu Y, Zhu S
Journal of pain research 2024;17:609-621
Journal of pain research 2024;17:609-621
Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia.
Yu S, Ye Y, Wuren T, Yi H
Frontiers in physiology 2024;15:1359357
Frontiers in physiology 2024;15:1359357
SPHK1 potentiates colorectal cancer progression and metastasis via regulating autophagy mediated by TRAF6-induced ULK1 ubiquitination.
Chen D, Wu J, Qiu X, Luo S, Huang S, Wei E, Qin M, Huang J, Liu S
Cancer gene therapy 2024 Mar;31(3):410-419
Cancer gene therapy 2024 Mar;31(3):410-419
Sphingosine kinase 1-specific inhibitor PF543 reduces goblet cell metaplasia of bronchial epithelium in an acute asthma model.
Sudhadevi T, Ackerman SJ, Jafri A, Basa P, Ha AW, Natarajan V, Harijith A
American journal of physiology. Lung cellular and molecular physiology 2024 Mar 1;326(3):L377-L392
American journal of physiology. Lung cellular and molecular physiology 2024 Mar 1;326(3):L377-L392
Protocol for the isolation of tumor cell-derived extracellular vesicles followed by in vivo metastasis assessment in a murine ovarian cancer model.
Mittal S, Kumar S, Gupta P, Singh M, Chaluvally-Raghavan P, Pradeep S
STAR protocols 2024 Jun 21;5(2):102943
STAR protocols 2024 Jun 21;5(2):102943
SPHK1/S1P/S1PR pathway promotes the progression of peritoneal fibrosis by mesothelial-mesenchymal transition.
Zhao T, Ding T, Sun Z, Shao X, Li S, Lu H, Yuan JH, Guo Z
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jan 31;38(2):e23417
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jan 31;38(2):e23417
ATF3 regulates SPHK1 in cardiomyocyte injury via endoplasmic reticulum stress.
Chen H, Luo S, Chen H, Zhang C
Immunity, inflammation and disease 2023 Sep;11(9):e998
Immunity, inflammation and disease 2023 Sep;11(9):e998
UCHL1 Regulates Radiation Lung Injury via Sphingosine Kinase-1.
Epshtein Y, Mathew B, Chen W, Jacobson JR
Cells 2023 Oct 5;12(19)
Cells 2023 Oct 5;12(19)
YTHDF1 mitigates acute kidney injury via safeguarding m(6)A-methylated mRNAs in stress granules of renal tubules.
Yang W, Zhang M, Li J, Qu S, Zhou F, Liu M, Li L, Liu Z, Zen K
Redox biology 2023 Nov;67:102921
Redox biology 2023 Nov;67:102921
Sphingosine Kinases at the Intersection of Pro-Inflammatory LPS and Anti-Inflammatory Endocannabinoid Signaling in BV2 Mouse Microglia Cells.
Standoli S, Rapino C, Di Meo C, Rudowski A, Kämpfer-Kolb N, Volk LM, Thomas D, Trautmann S, Schreiber Y, Meyer Zu Heringdorf D, Maccarrone M
International journal of molecular sciences 2023 May 9;24(10)
International journal of molecular sciences 2023 May 9;24(10)
Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer.
Zhang E, Ding C, Li S, Aikemu B, Zhou X, Fan X, Sun J, Yang X, Zheng M
Cancer cell international 2023 May 18;23(1):96
Cancer cell international 2023 May 18;23(1):96
A novel co-target of ACY1 governing plasma membrane translocation of SphK1 contributes to inflammatory and neuropathic pain.
Liu B, Wu W, Cui L, Zheng X, Li N, Zhang X, Duan G
iScience 2023 Jun 16;26(6):106989
iScience 2023 Jun 16;26(6):106989
The preventive effect of mussel oil on gestational diabetes mellitus in pregnant mice fed by a high-fat and high-sucrose diet.
Li K, Li H, Song X, Kuang X, Liu S, Zhu S, Li D
Food & function 2023 Jan 23;14(2):1198-1208
Food & function 2023 Jan 23;14(2):1198-1208
A comprehensive multiomics approach reveals that high levels of sphingolipids in cardiac cachexia adipose tissue are associated with inflammatory and fibrotic changes.
Qu Y, Wang Y, Wu T, Liu X, Wang H, Ma D
Lipids in health and disease 2023 Dec 1;22(1):211
Lipids in health and disease 2023 Dec 1;22(1):211
Establishment of a Rat Model of Liver Venous Deprivation: Simultaneous Portal and Hepatic Vein Ligation.
Zhang Y, He X, Ma P, Xiong L, Bai W, Zhang G, Xu Y, Song W, Yu K
Journal of clinical and translational hepatology 2023 Apr 28;11(2):393-404
Journal of clinical and translational hepatology 2023 Apr 28;11(2):393-404
LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion.
Zhao WJ, Fan YP, Ou GY, Qiao XY
Journal of Cancer 2022;13(7):2281-2292
Journal of Cancer 2022;13(7):2281-2292
Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2.
Liu JX, Liu M, Yu GZ, Zhao QQ, Wang JL, Sun YH, Koda S, Zhang B, Yu Q, Yan C, Tang RX, Jiang ZH, Zheng KY
Frontiers in cellular and infection microbiology 2022;12:1011378
Frontiers in cellular and infection microbiology 2022;12:1011378
Geniposide inhibits SphK1 membrane targeting to restore macrophage polarization balance in collagen-induced arthritis mice.
Gan PR, Wang RH, Deng R, Wu H, Bu YH, Chen FY, Dong XT, Ke JT
European journal of pharmacology 2022 Oct 15;933:175271
European journal of pharmacology 2022 Oct 15;933:175271
Metabolomic Analysis Reveals that SPHK1 Promotes Oral Squamous Cell Carcinoma Progression through NF-κB Activation.
Hou CX, Mao GY, Sun QW, Meng Y, Zhu QH, Tang YT, Han W, Sun NN, Song XM, Wang CX, Ye JH
Annals of surgical oncology 2022 Nov;29(12):7386-7399
Annals of surgical oncology 2022 Nov;29(12):7386-7399
Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.
Zhu J, Jin Z, Yang L, Zhao C, Hu J, Chen J, Han Y, Yu P, Luo J, Kong L, Zhang C
Pharmacological research 2022 Jun;180:106240
Pharmacological research 2022 Jun;180:106240
Berberine ameliorates erectile dysfunction in rats with streptozotocin-induced diabetes mellitus through the attenuation of apoptosis by inhibiting the SPHK1/S1P/S1PR2 and MAPK pathways.
Liu K, Sun T, Luan Y, Chen Y, Song J, Ling L, Yuan P, Li R, Cui K, Ruan Y, Lan R, Wang T, Wang S, Liu J, Rao K
Andrology 2022 Feb;10(2):404-418
Andrology 2022 Feb;10(2):404-418
Identifying the mechanisms and molecular targets of Hongjingtian injection on treatment of TGFβ1-induced HK-2 cells: coupling network pharmacology with experimental verification.
Sun G, Jiao M, Cui Y, Liang X, Liang X, Zhang S, Guo C
Annals of translational medicine 2022 Dec;10(24):1329
Annals of translational medicine 2022 Dec;10(24):1329
Proteomic-Based Approach Reveals the Involvement of Apolipoprotein A-I in Related Phenotypes of Autism Spectrum Disorder in the BTBR Mouse Model.
Li Q, Shi Y, Li X, Yang Y, Zhang X, Xu L, Ma Z, Wang J, Fan L, Wu L
International journal of molecular sciences 2022 Dec 4;23(23)
International journal of molecular sciences 2022 Dec 4;23(23)
CeO(2) nanoparticles induce pulmonary fibrosis via activating S1P pathway as revealed by metabolomics.
Cui L, Wang X, Zhao X, Sun B, Xia T, Hu S
Nano today 2022 Aug;45
Nano today 2022 Aug;45
Potential of a sphingosine 1-phosphate receptor antagonist and sphingosine kinase inhibitors as targets for multiple myeloma treatment.
Tanaka Y, Okabe S, Ohyashiki K, Gotoh A
Oncology letters 2022 Apr;23(4):111
Oncology letters 2022 Apr;23(4):111
Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture.
Pan G, Liao M, Dai Y, Li Y, Yan X, Mai W, Liu J, Liao Y, Qiu Z, Zhou Z
Frontiers in cardiovascular medicine 2021;8:748486
Frontiers in cardiovascular medicine 2021;8:748486
SphK2/S1P Promotes Metastasis of Triple-Negative Breast Cancer Through the PAK1/LIMK1/Cofilin1 Signaling Pathway.
Shi W, Ma D, Cao Y, Hu L, Liu S, Yan D, Zhang S, Zhang G, Wang Z, Wu J, Jiang C
Frontiers in molecular biosciences 2021;8:598218
Frontiers in molecular biosciences 2021;8:598218
SPHK1 contributes to cisplatin resistance in bladder cancer cells via the NONO/STAT3 axis.
Qin Z, Tong H, Li T, Cao H, Zhu J, Yin S, He W
International journal of molecular medicine 2021 Nov;48(5)
International journal of molecular medicine 2021 Nov;48(5)
Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.
Chen L, Ye A, Liu X, Lu J, Xie Q, Guo Y, Sun W
Ecotoxicology and environmental safety 2021 Aug 26;224:112704
Ecotoxicology and environmental safety 2021 Aug 26;224:112704
Bone marrow mesenchymal stem cells-derived exosomes reduce Aβ deposition and improve cognitive function recovery in mice with Alzheimer's disease by activating sphingosine kinase/sphingosine-1-phosphate signaling pathway.
Wang X, Yang G
Cell biology international 2021 Apr;45(4):775-784
Cell biology international 2021 Apr;45(4):775-784
POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling.
Shen Z, Feng X, Fang Y, Li Y, Li Z, Zhan Y, Lin M, Li G, Ding Y, Deng H
Cell death & disease 2019 Nov 13;10(11):863
Cell death & disease 2019 Nov 13;10(11):863
Sphingosine kinase 1 knockout alleviates hepatic ischemia/reperfusion injury by attenuating inflammation and oxidative stress in mice.
Qiang GH, Wang ZX, Ji AL, Wu JY, Cao Y, Zhang G, Zhang YY, Jiang CP
Hepatobiliary & pancreatic diseases international : HBPD INT 2019 Jun;18(3):255-265
Hepatobiliary & pancreatic diseases international : HBPD INT 2019 Jun;18(3):255-265
Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.
Xiao J, Lin H, Liu B, Xia Z, Zhang J, Jin J
Biomarkers in medicine 2019 Jun;13(8):627-637
Biomarkers in medicine 2019 Jun;13(8):627-637
Sphingosine kinase 1 promotes the metastasis of colorectal cancer by inducing the epithelial‑mesenchymal transition mediated by the FAK/AKT/MMPs axis.
Liu SQ, Xu CY, Wu WH, Fu ZH, He SW, Qin MB, Huang JA
International journal of oncology 2019 Jan;54(1):41-52
International journal of oncology 2019 Jan;54(1):41-52
Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.
Pang HQ, Yue SJ, Tang YP, Chen YY, Tan YJ, Cao YJ, Shi XQ, Zhou GS, Kang A, Huang SL, Shi YJ, Sun J, Tang ZS, Duan JA
Frontiers in pharmacology 2018;9:165
Frontiers in pharmacology 2018;9:165
Bidirectional effects of moxifloxacin on the pro‑inflammatory response in lipopolysaccharide‑stimulated mouse peritoneal macrophages.
Qiu Z, Yuan H, Li N, Yang X, Hu X, Su F, Chen B
Molecular medicine reports 2018 Dec;18(6):5399-5408
Molecular medicine reports 2018 Dec;18(6):5399-5408
SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells.
Xu CY, Liu SQ, Qin MB, Zhuge CF, Qin L, Qin N, Lai MY, Huang JA
International journal of molecular medicine 2017 May;39(5):1277-1284
International journal of molecular medicine 2017 May;39(5):1277-1284
Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1).
Lu Z, Xiao Z, Liu F, Cui M, Li W, Yang Z, Li J, Ye L, Zhang X
Oncotarget 2016 Jan 5;7(1):241-54
Oncotarget 2016 Jan 5;7(1):241-54
ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.
Liu X, Ren K, Suo R, Xiong SL, Zhang QH, Mo ZC, Tang ZL, Jiang Y, Peng XS, Yi GH
Journal of physiology and biochemistry 2016 Dec;72(4):657-667
Journal of physiology and biochemistry 2016 Dec;72(4):657-667
Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1.
Lu ZP, Xiao ZL, Yang Z, Li J, Feng GX, Chen FQ, Li YH, Feng JY, Gao YE, Ye LH, Zhang XD
Acta pharmacologica Sinica 2015 Oct;36(10):1228-36
Acta pharmacologica Sinica 2015 Oct;36(10):1228-36
MiR-506 suppresses liver cancer angiogenesis through targeting sphingosine kinase 1 (SPHK1) mRNA.
Lu Z, Zhang W, Gao S, Jiang Q, Xiao Z, Ye L, Zhang X
Biochemical and biophysical research communications 2015 Dec 4-11;468(1-2):8-13
Biochemical and biophysical research communications 2015 Dec 4-11;468(1-2):8-13
Phosphorylation and action of the immunomodulator FTY720 inhibits vascular endothelial cell growth factor-induced vascular permeability.
Sanchez T, Estrada-Hernandez T, Paik JH, Wu MT, Venkataraman K, Brinkmann V, Claffey K, Hla T
The Journal of biological chemistry 2003 Nov 21;278(47):47281-90
The Journal of biological chemistry 2003 Nov 21;278(47):47281-90
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SPHK1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SPHK1. This antibody recognizes human,mouse,rat antigen. The SPHK1 antibody has been validated for the following applications: ELISA, IHC analysis.
