AP7237c

antibody from Abcepta

Targeting: SPHK1 SPHK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7237c

Provider

Abcepta

Proper citation

Abgent Cat#AP7237c, RRID:AB_2195814

Product name

SPHK1 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions.

Fischer I, Alliod C, Martinier N, Newcombe J, Brana C, Pouly S
PloS one 2011;6(8):e23905

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Isoflurane activates intestinal sphingosine kinase to protect against bilateral nephrectomy-induced liver and intestine dysfunction.

Kim M, Park SW, Kim M, D'Agati VD, Lee HT
American journal of physiology. Renal physiology 2011 Jan;300(1):F167-76

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Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury.

Kim M, Park SW, Kim M, D'Agati VD, Lee HT
Anesthesiology 2011 Feb;114(2):363-73

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Sphingosine kinase-1 enhances resistance to apoptosis through activation of PI3K/Akt/NF-κB pathway in human non-small cell lung cancer.

Song L, Xiong H, Li J, Liao W, Wang L, Wu J, Li M
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Apr 1;17(7):1839-49

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Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy.

Liu G, Zheng H, Zhang Z, Wu Z, Xiong H, Li J, Song L
BMC cancer 2010 Sep 16;10:495

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Sphingosine kinase 1 regulates the expression of proinflammatory cytokines and nitric oxide in activated microglia.

Nayak D, Huo Y, Kwang WX, Pushparaj PN, Kumar SD, Ling EA, Dheen ST
Neuroscience 2010 Mar 10;166(1):132-44

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Sphingosine kinase 1 is associated with gastric cancer progression and poor survival of patients.

Li W, Yu CP, Xia JT, Zhang L, Weng GX, Zheng HQ, Kong QL, Hu LJ, Zeng MS, Zeng YX, Li M, Li J, Song LB
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Feb 15;15(4):1393-9

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Sphingosine-1-phosphate elicits receptor-dependent calcium signaling in retinal amacrine cells.

Crousillac S, Colonna J, McMains E, Dewey JS, Gleason E
Journal of neurophysiology 2009 Dec;102(6):3295-309

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Differential regulation of sphingosine kinases 1 and 2 in lung injury.

Wadgaonkar R, Patel V, Grinkina N, Romano C, Liu J, Zhao Y, Sammani S, Garcia JG, Natarajan V
American journal of physiology. Lung cellular and molecular physiology 2009 Apr;296(4):L603-13

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Clinical significance of sphingosine kinase-1 expression in human astrocytomas progression and overall patient survival.

Li J, Guan HY, Gong LY, Song LB, Zhang N, Wu J, Yuan J, Zheng YJ, Huang ZS, Li M
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Nov 1;14(21):6996-7003

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Activation of sphingosine kinase-1 mediates inhibition of vascular smooth muscle cell apoptosis by hyperglycemia.

You B, Ren A, Yan G, Sun J
Diabetes 2007 May;56(5):1445-53

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FHL2/SLIM3 decreases cardiomyocyte survival by inhibitory interaction with sphingosine kinase-1.

Sun J, Yan G, Ren A, You B, Liao JK
Circulation research 2006 Sep 1;99(5):468-76

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301)(Cat. #AP7237c). AP7237c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of anti-SPHK1 Antibody (Center) (Cat.#AP7237c) in 293 cell line lysates (35ug/lane). SPHK1 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301) (Cat.#AP7237c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100