ARG55845

antibody from Arigo

Targeting: SPHK1 SPHK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG55845

Provider

Arigo

Product name

anti-SPHK1 antibody

Antibody type

Polyclonal

Antigen

KLH-conjugated synthetic peptide around aa. 286-315 (Center) of Human SPHK1.

Description

Purification with Protein G.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 35 µg of 293 cell line lysates stained with ARG55845 anti-SPHK1 antibody.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 2 µg of 293 cell lysates either 1) nontransfected or 2) transiently transfected with the SPHK1 gene stained with ARG55845 anti-SPHK1 antibody.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 35 µg of 293, HepG2, HUVEC, Raji, Rat C6, Mouse C2C12 cell line (from left to right) stained with ARG55845 anti-SPHK1 antibody at 1:1000 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: formalin-fixed and paraffin-embedded Human cancer tissue stained with ARG55845 anti-SPHK1 antibody.