Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN359438 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Sphingosine Kinase 1 (SPHK1) (AA 286-315) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of Human SPHK1.
- Description
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Epitope
- AA 286-315
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Sphingosine kinase 1 is associated with gastric cancer progression and poor survival of patients.
Complete sequencing and characterization of 21,243 full-length human cDNAs.
Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes.
Functional characterization of human sphingosine kinase-1.
Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution.
Li W, Yu CP, Xia JT, Zhang L, Weng GX, Zheng HQ, Kong QL, Hu LJ, Zeng MS, Zeng YX, Li M, Li J, Song LB
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Feb 15;15(4):1393-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Feb 15;15(4):1393-9
Complete sequencing and characterization of 21,243 full-length human cDNAs.
Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S
Nature genetics 2004 Jan;36(1):40-5
Nature genetics 2004 Jan;36(1):40-5
Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes.
Pitson SM, D'andrea RJ, Vandeleur L, Moretti PA, Xia P, Gamble JR, Vadas MA, Wattenberg BW
The Biochemical journal 2000 Sep 1;350 Pt 2:429-41
The Biochemical journal 2000 Sep 1;350 Pt 2:429-41
Functional characterization of human sphingosine kinase-1.
Nava VE, Lacana E, Poulton S, Liu H, Sugiura M, Kono K, Milstien S, Kohama T, Spiegel S
FEBS letters 2000 May 4;473(1):81-4
FEBS letters 2000 May 4;473(1):81-4
Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution.
Melendez AJ, Carlos-Dias E, Gosink M, Allen JM, Takacs L
Gene 2000 Jun 13;251(1):19-26
Gene 2000 Jun 13;251(1):19-26
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- The anti-SPHK1 Pab (AP13922PU-N) is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of anti-SPHK1 Antibody (Center) (AP13922PU-N) in 293 cell line lysates (35 μg/lane). SPHK1 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.