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- References [20]
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- Product number
- 19561-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#19561-1-AP, RRID:AB_10950399
- Product name
- Phospho-SPHK1 (Ser225) antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Phospho-SPHK1 (Ser225) antibody (Cat. #19561-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 100ul
Submitted references The Combination of PF-543 and TRAIL Effectively Induces Apoptotic Cell Death and Inhibits Stem Cell-Like Properties Through the SPHK1/S1PR1/STAT3 Pathway in TRAIL-Resistant Colorectal Cancer Cells.
Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis.
Polycyclic aromatic hydrocarbon aggravates high-fat diet-induced metabolic dysfunction-associated steatotic liver disease through disturbing hepatocyte sphingolipid metabolism.
Transcriptional signature of rapidly responding NK cells reveals S1P5 and CXCR4 as anti-tumor response disruptors.
The new anti-angiogenesis perspective of rheumatoid arthritis with geniposide: Reducing the extracellular release of HSP70 in HUVECs.
Autophagy-dependent secretion of ENO1 mediates chemoresistance of glioblastoma and tumor microenvironment remodeling.
Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia.
Targeting the SphK1/S1P/PFKFB3 axis suppresses hepatocellular carcinoma progression by disrupting glycolytic energy supply that drives tumor angiogenesis.
Geniposide inhibits SphK1 membrane targeting to restore macrophage polarization balance in collagen-induced arthritis mice.
Growth Inhibitory and Pro-Apoptotic Effects of Hirsuteine in Chronic Myeloid Leukemia Cells through Targeting Sphingosine Kinase 1.
Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.
Endogenous Ca(2+) release was involved in 50-Hz MF-induced proliferation via Akt-SK1 signal cascade in human amniotic epithelial cells.
Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.
Cell fate determined by the activation balance between PKR and SPHK1.
Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro.
A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK-S1P-S1PR cascade mediated ERK signaling pathway.
POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling.
Imbalanced sphingolipid signaling is maintained as a core proponent of a cancerous phenotype in spite of metabolic pressure and epigenetic drift.
Involvement of released sphingosine 1-phosphate/sphingosine 1-phosphate receptor axis in skeletal muscle atrophy.
Cross talk between MMP2-Spm-Cer-S1P and ERK1/2 in proliferation of pulmonary artery smooth muscle cells under angiotensin II stimulation.
Kim SL, Shin MW, Jin BC, Seo SY, Kim SW
Digestive diseases and sciences 2025 Sep;70(9):3041-3055
Digestive diseases and sciences 2025 Sep;70(9):3041-3055
Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis.
Zhan Y, Xu J, Zhang Z, Hu Y, Li Y, Qian J, Ling Y, Wu D, Deng H, Li G, Shen Z, Fang Y
Cancer communications (London, England) 2025 Oct;45(10):1203-1228
Cancer communications (London, England) 2025 Oct;45(10):1203-1228
Polycyclic aromatic hydrocarbon aggravates high-fat diet-induced metabolic dysfunction-associated steatotic liver disease through disturbing hepatocyte sphingolipid metabolism.
Kuo HR, Chung YF, Wang LT, Wang CW, Hsu SH, Chen LC, Lin MH, Huang SK, Liu KY
Environmental pollution (Barking, Essex : 1987) 2025 Oct 15;383:126846
Environmental pollution (Barking, Essex : 1987) 2025 Oct 15;383:126846
Transcriptional signature of rapidly responding NK cells reveals S1P5 and CXCR4 as anti-tumor response disruptors.
Puig-Gámez M, Van Attekum M, Theis T, Dick A, Park JE
Scientific reports 2025 Mar 28;15(1):10769
Scientific reports 2025 Mar 28;15(1):10769
The new anti-angiogenesis perspective of rheumatoid arthritis with geniposide: Reducing the extracellular release of HSP70 in HUVECs.
Wei Y, Li Y, Shu Y, Gan PR, Zhu YL, Xu J, Jiang XM, Xia SL, Wang Y, Wu H
International immunopharmacology 2025 Jan 10;144:113645
International immunopharmacology 2025 Jan 10;144:113645
Autophagy-dependent secretion of ENO1 mediates chemoresistance of glioblastoma and tumor microenvironment remodeling.
Xie Q, Chen L, Huang Y, Yu Z, Zhu R, Li J, Zhao J, Song Y, Li H, Lu Y
Cell death & disease 2025 Dec 6;17(1):79
Cell death & disease 2025 Dec 6;17(1):79
Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia.
Yu S, Ye Y, Wuren T, Yi H
Frontiers in physiology 2024;15:1359357
Frontiers in physiology 2024;15:1359357
Targeting the SphK1/S1P/PFKFB3 axis suppresses hepatocellular carcinoma progression by disrupting glycolytic energy supply that drives tumor angiogenesis.
Liu XT, Huang Y, Liu D, Jiang YC, Zhao M, Chung LH, Han XD, Zhao Y, Chen J, Coleman P, Ting KK, Tran C, Su Y, Dennis CV, Bhatnagar A, Liu K, Don AS, Vadas MA, Gorrell MD, Zhang S, Murray M, Kavurma MM, McCaughan GW, Gamble JR, Qi Y
Journal of translational medicine 2024 Jan 10;22(1):43
Journal of translational medicine 2024 Jan 10;22(1):43
Geniposide inhibits SphK1 membrane targeting to restore macrophage polarization balance in collagen-induced arthritis mice.
Gan PR, Wang RH, Deng R, Wu H, Bu YH, Chen FY, Dong XT, Ke JT
European journal of pharmacology 2022 Oct 15;933:175271
European journal of pharmacology 2022 Oct 15;933:175271
Growth Inhibitory and Pro-Apoptotic Effects of Hirsuteine in Chronic Myeloid Leukemia Cells through Targeting Sphingosine Kinase 1.
Gao S, Guo T, Luo S, Zhang Y, Ren Z, Lang X, Hu G, Zuo D, Jia W, Kong D, Yu H, Qiu Y
Biomolecules & therapeutics 2022 Nov 1;30(6):553-561
Biomolecules & therapeutics 2022 Nov 1;30(6):553-561
Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.
Wang Y, Wu H, Gui BJ, Liu J, Rong GX, Deng R, Bu YH, Zhang H
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jun;100:154068
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jun;100:154068
Endogenous Ca(2+) release was involved in 50-Hz MF-induced proliferation via Akt-SK1 signal cascade in human amniotic epithelial cells.
Ye AF, Liu XC, Chen LJ, Xia YP, Yang XB, Sun WJ
Electromagnetic biology and medicine 2022 Apr 3;41(2):142-151
Electromagnetic biology and medicine 2022 Apr 3;41(2):142-151
Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.
Chen L, Ye A, Liu X, Lu J, Xie Q, Guo Y, Sun W
Ecotoxicology and environmental safety 2021 Nov;224:112704
Ecotoxicology and environmental safety 2021 Nov;224:112704
Cell fate determined by the activation balance between PKR and SPHK1.
Qiao H, Jiang T, Mu P, Chen X, Wen X, Hu Z, Tang S, Wen J, Deng Y
Cell death and differentiation 2021 Jan;28(1):401-418
Cell death and differentiation 2021 Jan;28(1):401-418
Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro.
Wang Y, Wu H, Deng R, Dai XJ, Bu YH, Sun MH, Zhang H, Wang MD, Wang RH
Phytotherapy research : PTR 2021 Aug;35(8):4347-4362
Phytotherapy research : PTR 2021 Aug;35(8):4347-4362
A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK-S1P-S1PR cascade mediated ERK signaling pathway.
Chen L, Xia Y, Lu J, Xie Q, Ye A, Sun W
Ecotoxicology and environmental safety 2020 May;194:110407
Ecotoxicology and environmental safety 2020 May;194:110407
POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling.
Shen Z, Feng X, Fang Y, Li Y, Li Z, Zhan Y, Lin M, Li G, Ding Y, Deng H
Cell death & disease 2019 Nov 13;10(11):863
Cell death & disease 2019 Nov 13;10(11):863
Imbalanced sphingolipid signaling is maintained as a core proponent of a cancerous phenotype in spite of metabolic pressure and epigenetic drift.
Speirs MMP, Swensen AC, Chan TY, Jones PM, Holman JC, Harris MB, Maschek JA, Cox JE, Carson RH, Hill JT, Andersen JL, Prince JT, Price JC
Oncotarget 2019 Jan 11;10(4):449-479
Oncotarget 2019 Jan 11;10(4):449-479
Involvement of released sphingosine 1-phosphate/sphingosine 1-phosphate receptor axis in skeletal muscle atrophy.
Pierucci F, Frati A, Battistini C, Matteini F, Iachini MC, Vestri A, Penna F, Costelli P, Meacci E
Biochimica et biophysica acta. Molecular basis of disease 2018 Dec;1864(12):3598-3614
Biochimica et biophysica acta. Molecular basis of disease 2018 Dec;1864(12):3598-3614
Cross talk between MMP2-Spm-Cer-S1P and ERK1/2 in proliferation of pulmonary artery smooth muscle cells under angiotensin II stimulation.
Chowdhury A, Sarkar J, Pramanik PK, Chakraborti T, Chakraborti S
Archives of biochemistry and biophysics 2016 Aug 1;603:91-101
Archives of biochemistry and biophysics 2016 Aug 1;603:91-101
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SPHK1-Phospho-Ser225 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human SPHK1-Phospho-Ser225. This antibody recognizes human antigen. The SPHK1-Phospho-Ser225 antibody has been validated for the following applications: ELISA, WB analysis.
