Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-B2724 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B2724, RRID:AB_2059225
- Product name
- IHC-plus™ ATG16L1 / ATG16L Antibody LS-B2724
- Antibody type
- Polyclonal
- Description
- Peptide Column Affinity Chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of Anti-ATG16L1 antibody (LS-B2724, 1µg/ml; 15 µg of lysate per lane). Lane 1: A431 cell line. Lane 2: A549 cell line. Lane 3: Caco2 cell line. Antibody produced bands at ~68 kDa (Isoform 1), and ~66 kDa (isoform 2)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot of ATG16 in HeLa cell lysate with ATG16 antibody at (A) 0.25 and (B) 0.5 ug/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of Anti-ATG16L1 antibody (LS-B2724, 1µg/ml; 15 µg of lysate per lane). Lane 1: A431 cell line. Lane 2: A549 cell line. Lane 3: Caco2 cell line. Antibody produced bands at ~68 kDa (Isoform 1), and ~66 kDa (isoform 2)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 ug/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 ug/ml.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-ATG16L1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-ATG16L1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.