PA1-18296

antibody from Invitrogen Antibodies

Targeting: ATG16L1 APG16L, ATG16A, ATG16L, FLJ10035, WDR30

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-18296

Provider

Invitrogen Antibodies

Product name

ATG16L1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

The Lipid Virulence Factors of Mycobacterium tuberculosis Exert Multilayered Control over Autophagy-Related Pathways in Infected Human Macrophages.

Bah A, Sanicas M, Nigou J, Guilhot C, Astarie-Dequeker C, Vergne I
Cells 2020 Mar 9;9(3)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 The Mtb mutant deficient in DIMs and SLs promoted autophagy activation. ( A - D ) Differentiated THP-1 cells were infected for 1 h with the indicated GFP-expressing Mtb strains; fixed at 48 h, 72 h, or 96 h postinfection (MOI between 5 and 10); permeabilized; incubated with antibodies against endogenous Beclin-1 ( A , C ) or Atg16L1 ( B , D ); and then stained with Alexa-568-labeled secondary antibody. Specimens were analyzed using confocal fluorescence microscopy. ( A , B ) Representative confocal images of macrophages infected with GFP-expressing Mtb (green channel) at 48 h postinfection, stained for Beclin-1 ( A ) or Atg16L1 ( B ) (red channel). The boxed areas in the left-hand panels are magnifications of the right-hand panels. Scale bars are 10 mum (left panels) or 2 mum (right panels). ( C , D ) A quantification of the percentage of Mtb compartments colocalized with Beclin-1 ( C ) or Atg16L1 ( D ) at 48 h, 72 h, and 96 h postinfection. Data are the mean +- s.e.m; 90 fields from three independent experiments; *** p < 0.0001 (unpaired t -test). ( E ) Differentiated THP-1 cells were infected for 1 h with the indicated Mtb strains and lysed 48 h postinfection. Cells were treated with 100 nM Bafilomycin A1 (with BafA1) or a DMSO (dimethylsulfoxide) (control (without BafA1) during the last 2 h postinfection. Lysates were analyzed using immunoblotting with anti-LC3 or anti-actin antibodies. The densitometric LC3-II/actin ratios are shown underneath the blot. The ratio