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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP1817d - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1817d, RRID:AB_2059222
- Product name
- ATG16L Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant Protein
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Autophagy in cancer associated fibroblasts promotes tumor cell survival: Role of hypoxia, HIF1 induction and NFκB activation in the tumor stromal microenvironment.
An American College of Surgeons response to the ODHS survey.
Martinez-Outschoorn UE, Trimmer C, Lin Z, Whitaker-Menezes D, Chiavarina B, Zhou J, Wang C, Pavlides S, Martinez-Cantarin MP, Capozza F, Witkiewicz AK, Flomenberg N, Howell A, Pestell RG, Caro J, Lisanti MP, Sotgia F
Cell cycle (Georgetown, Tex.) 2010 Sep 1;9(17):3515-33
Cell cycle (Georgetown, Tex.) 2010 Sep 1;9(17):3515-33
An American College of Surgeons response to the ODHS survey.
Reiling RB
Ohio medicine : journal of the Ohio State Medical Association 1988 Jul;84(7):576-8
Ohio medicine : journal of the Ohio State Medical Association 1988 Jul;84(7):576-8
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-APG16 Pab (Cat.#AP1817d) in Hela cell line lysates (35ug/lane).APG16(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 ¼M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817d ATG16L primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978)."
- Primary Ab dilution
- 1:100
