AP1817d

antibody from Abcepta

Targeting: ATG16L1 APG16L, ATG16A, ATG16L, FLJ10035, WDR30

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1817d

Provider

Abcepta

Proper citation

Abgent Cat#AP1817d, RRID:AB_2059222

Product name

ATG16L Antibody

Antibody type

Polyclonal

Antigen

Recombinant Protein

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Autophagy in cancer associated fibroblasts promotes tumor cell survival: Role of hypoxia, HIF1 induction and NFκB activation in the tumor stromal microenvironment.

Martinez-Outschoorn UE, Trimmer C, Lin Z, Whitaker-Menezes D, Chiavarina B, Zhou J, Wang C, Pavlides S, Martinez-Cantarin MP, Capozza F, Witkiewicz AK, Flomenberg N, Howell A, Pestell RG, Caro J, Lisanti MP, Sotgia F
Cell cycle (Georgetown, Tex.) 2010 Sep 1;9(17):3515-33

---

An American College of Surgeons response to the ODHS survey.

Reiling RB
Ohio medicine : journal of the Ohio State Medical Association 1988 Jul;84(7):576-8

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of anti-APG16 Pab (Cat.#AP1817d) in Hela cell line lysates (35ug/lane).APG16(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 ¼M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817d ATG16L primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978)."

Primary Ab dilution

1:100