Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARG54957 - Provider product page
- Provider
- Arigo
- Product name
- anti-ATG16L antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 161-190 from Human ATG16L.
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of NCI-H460 cell line lysates stained with ARG54957 anti-ATG16L antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 1) Cos7, 2) HEK293, 3) MEF, and 4) HeLa cells stained with ARG54957 anti-ATG16L antibody. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cells stained with ARG54957 anti-ATG16L antibody (green) at 1:25 dilution. DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded human colon carcinoma tissue stained with ARG54957 anti-ATG16L antibody.