ARG55141

antibody from Arigo

Targeting: ATG16L1 APG16L, ATG16A, ATG16L, FLJ10035, WDR30

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG55141

Provider

Arigo

Product name

anti-ATG16L antibody

Antibody type

Polyclonal

Antigen

KLH-conjugated synthetic peptide from Human ATG16L.

Description

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

200 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 35 µg of HeLa cell line lysates stained with ARG55141 anti-ATG16L antibody.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: U251 cells stained with ARG55141 anti-ATG16L antibody (green) at 1:100 dilution, 2 h at room temperature. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ARG55141 anti-ATG16L antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978).