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LearnABIN1449612
antibody from antibodies-online
Targeting: ATG16L1
APG16L, ATG16A, ATG16L, FLJ10035, WDR30
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- ABIN1449612 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-ATG16 Autophagy Related 16-Like 1 (S. Cerevisiae) (ATG16L1) (AA 84-114) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 84~114 amino acids surrounding amino acid L92 of Human APG16L Genename: ATG16L1
- Description
- Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 84-114
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease.
Nod1 and Nod2 direct autophagy by recruiting ATG16L1 to the plasma membrane at the site of bacterial entry.
Overexpression and altered subcellular localization of autophagy-related 16-like 1 in human oral squamous-cell carcinoma: correlation with lymphovascular invasion and lymph-node metastasis.
Degrade or die: a dual function for autophagy in the plant immune response.
Autophagy: dual roles in life and death?
Autophagy in metazoans: cell survival in the land of plenty.
Eating oneself and uninvited guests: autophagy-related pathways in cellular defense.
Autophagy in health and disease: a double-edged sword.
Nascimento-Ferreira I, Santos-Ferreira T, Sousa-Ferreira L, Auregan G, Onofre I, Alves S, Dufour N, Colomer Gould VF, Koeppen A, Déglon N, Pereira de Almeida L
Brain : a journal of neurology 2011 May;134(Pt 5):1400-15
Brain : a journal of neurology 2011 May;134(Pt 5):1400-15
Nod1 and Nod2 direct autophagy by recruiting ATG16L1 to the plasma membrane at the site of bacterial entry.
Travassos LH, Carneiro LA, Ramjeet M, Hussey S, Kim YG, Magalhães JG, Yuan L, Soares F, Chea E, Le Bourhis L, Boneca IG, Allaoui A, Jones NL, Nuñez G, Girardin SE, Philpott DJ
Nature immunology 2010 Jan;11(1):55-62
Nature immunology 2010 Jan;11(1):55-62
Overexpression and altered subcellular localization of autophagy-related 16-like 1 in human oral squamous-cell carcinoma: correlation with lymphovascular invasion and lymph-node metastasis.
Nomura H, Uzawa K, Yamano Y, Fushimi K, Ishigami T, Kouzu Y, Koike H, Siiba M, Bukawa H, Yokoe H, Kubosawa H, Tanzawa H
Human pathology 2009 Jan;40(1):83-91
Human pathology 2009 Jan;40(1):83-91
Degrade or die: a dual function for autophagy in the plant immune response.
Greenberg JT
Developmental cell 2005 Jun;8(6):799-801
Developmental cell 2005 Jun;8(6):799-801
Autophagy: dual roles in life and death?
Baehrecke EH
Nature reviews. Molecular cell biology 2005 Jun;6(6):505-10
Nature reviews. Molecular cell biology 2005 Jun;6(6):505-10
Autophagy in metazoans: cell survival in the land of plenty.
Lum JJ, DeBerardinis RJ, Thompson CB
Nature reviews. Molecular cell biology 2005 Jun;6(6):439-48
Nature reviews. Molecular cell biology 2005 Jun;6(6):439-48
Eating oneself and uninvited guests: autophagy-related pathways in cellular defense.
Levine B
Cell 2005 Jan 28;120(2):159-62
Cell 2005 Jan 28;120(2):159-62
Autophagy in health and disease: a double-edged sword.
Shintani T, Klionsky DJ
Science (New York, N.Y.) 2004 Nov 5;306(5698):990-5
Science (New York, N.Y.) 2004 Nov 5;306(5698):990-5
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis using APG16L Antibody Cat.-No AP32180PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the APG16L antibody detected the APG16L protein (arrow).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis using APG16L Antibody Cat.-No AP32180PU-N in Mouse brain tissue lysate.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis using APG16L Antibody Cat.-No AP32180PU-N in Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L Antibody Cat.-No AP32180PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed paraffin-embedded biopsy from patient with confirmed colonic Crohn disease. Slide subjected to a citrate-based antigen retrieval procedure, permeabilized by incubation with 0.1% Triton X-100 in 0.1M PBS) washed three times in PBS and blocked with 0.75% bovine serum albumin in PBS for 20 minutes. Sections subsequently incubated with APG16L primary antibody at a 1/200 dilution in 0.75% BSA for 1 h RT. Data courtesy of J. Hampe, S. Schreiber, P. Rosenstiel et al., Institute for Clinical Molecular Biology and First Department of Medicine, Christian-Albrechts University Kiel, University Hospital Schleswig-Holstein, 24105 Kiel, Germany.
