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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-31150 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRYZ Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, Jurkat, Raji, mouse brain. Predicted reactivity: Human (99%), Mouse (81%), Rat (82%), Dog (87%), Pig (80%), Rhesus Monkey (96%), Bovine (87%), Guinea pig (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CRYZ Polyclonal Antibody (Product # PA5-31150). Sample (30 µg of whole cell lysate). Lane A: Jurkat. Lane B: Raji. 10% SDS PAGE. CRYZ Polyclonal Antibody (Product # PA5-31150) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CRYZ Polyclonal Antibody (Product # PA5-31150). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. CRYZ Polyclonal Antibody (Product # PA5-31150) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- zeta Crystallin antibody [N1C1] detects zeta Crystallin protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: zeta Crystallin protein stained by zeta Crystallin antibody [N1C1] (Product # PA5-31150) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using zeta Crystallin (Product # PA5-31150) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
