12135-1-AP

antibody from Proteintech Group

Targeting: MAP1LC3A ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3

Provider product page for 12135-1-AP

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Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [69]
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Western blot [1]

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Product number: 12135-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12135-1-AP, RRID:AB_2281381
Product name: LC3 antibody
Antibody type: Polyclonal
Description: LC3 antibody (Cat. #12135-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, zebrafish and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat, Zebrafish
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MAP1LC3A protein structure - 12135-1-AP shown in red.

Submitted references The interlacing anticancer effect of pharmacologic ascorbate, chloroquine, and resveratrol.

Makk-Merczel K, Varga D, Hajdinák P, Szarka A
BioFactors (Oxford, England) 2024 Sep-Oct;50(5):980-996

Co-exposure of arsenic and polystyrene-nanoplastics induced kidney injury by disrupting mitochondrial homeostasis and mtROS-mediated ferritinophagy and ferroptosis.

Zhong G, Qiao B, He Y, Liu H, Hong P, Rao G, Tang L, Tang Z, Hu L
Pesticide biochemistry and physiology 2024 May;201:105904

Targeting ENPP1 depletion may be a promising therapeutic strategy for treating oral squamous cell carcinoma via cytotoxic autophagy-related apoptosis.

Ma C, Zhao J, Zhou L, Jia C, Shi Y, Li X, Jihu K, Zhang T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jan 31;38(2):e23420

Geniposide effectively safeguards HT22 cells against Aβ-induced damage by activating mitophagy via the PINK1/Parkin signaling pathway.

Ye J, Wu J, Ai L, Zhu M, Li Y, Yin D, Huang Q
Biochemical pharmacology 2024 Aug;226:116296

Effect of Nano-selenium on Biological Mechanism of Goblet Cells of the Small Intestine Within Laying Hen.

Yin Y, Ahmed N, Hassan MF, Guo K, Shakir Y, Zang J, Lyu J
Biological trace element research 2024 Apr;202(4):1699-1710

Analysis of ATG4C function in vivo.

Tamargo-Gómez I, Martínez-García GG, Suárez MF, Mayoral P, Bretones G, Astudillo A, Prieto-Lloret J, Sveen C, Fueyo A, Engedal N, López-Otín C, Mariño G
Autophagy 2023 Nov;19(11):2912-2933

Balance between autophagy and cell death is maintained by Polycomb-mediated regulation during stem cell differentiation.

Puri D, Kelkar A, Gaurishankar B, Subramanyam D
The FEBS journal 2023 Mar;290(6):1625-1644

Poricoic acid A induces mitophagy to ameliorate podocyte injury in diabetic kidney disease via downregulating FUNDC1.

Wu Y, Deng H, Sun J, Tang J, Li X, Xu Y
Journal of biochemical and molecular toxicology 2023 Dec;37(12):e23503

Cadmium-induced impairment of spermatozoa development by reducing exosomal-MVBs secretion: a novel pathway.

Ali W, Bian Y, Ali H, Sun J, Zhu J, Ma Y, Liu Z, Zou H
Aging 2023 Apr 25;15(10):4096-4107

Mesangial Cell-Derived Exosomal miR-4455 Induces Podocyte Injury in IgA Nephropathy by Targeting ULK2.

Yu M, Shen X, He W, Zheng D, He Q, Jin J
Oxidative medicine and cellular longevity 2022;2022:1740770

Liuwei Dihuang Pills Inhibit Podocyte Injury and Alleviate IgA Nephropathy by Directly Altering Mesangial Cell-Derived Exosome Function and Secretion.

Zhu X, Shen X, Lin B, Fang J, Jin J, He Q
Frontiers in pharmacology 2022;13:889008

PIN1 protects auditory hair cells from senescence via autophagy.

Lv Z, Zhang Y, Cao H, Liu Q, Feng X, Yin H, Wang B
PeerJ 2022;10:e14267

A racemosin B derivative, C25, suppresses breast cancer growth via lysosomal membrane permeabilization and inhibition of autophagic flux.

Xiao X, Chung PED, Xu M, Hu A, Ju Y, Yang X, Song J, Song J, Wang C, Zacksenhaus E, Liu S, He Z, Ben-David Y
Biochemical pharmacology 2022 Jul;201:115060

S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.

Zhang L, Zhang Z, Li C, Zhu T, Gao J, Zhou H, Zheng Y, Chang Q, Wang M, Wu J, Ran L, Wu Y, Miao H, Zou X, Liang B
Cellular and molecular gastroenterology and hepatology 2021;11(3):697-724

ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration.

Tamargo-Gómez I, Martínez-García GG, Suárez MF, Rey V, Fueyo A, Codina-Martínez H, Bretones G, Caravia XM, Morel E, Dupont N, Cabo R, Tomás-Zapico C, Souquere S, Pierron G, Codogno P, López-Otín C, Fernández ÁF, Mariño G
Cell death and differentiation 2021 Sep;28(9):2651-2672

Activated FMS-like tyrosine kinase 3 ameliorates angiotensin II-induced cardiac remodelling.

Ma W, Liang F, Zhan H, Jiang X, Gao C, Zhang X, Zhang K, Sun Q, Hu H, Zhao Z
Acta physiologica (Oxford, England) 2020 Oct;230(2):e13519

Drp1-mediated mitochondrial fission induced autophagy attenuates cell apoptosis caused by 3-chlorpropane-1,2-diol in HEK293 cells.

Jin C, Zhong Y, Han J, Zhu J, Liu Q, Sun D, Xia X, Peng X
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2020 Nov;145:111740

Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress.

Tian Y, Guo H, Miao X, Xu J, Yang R, Zhao L, Liu J, Yang L, Gao F, Zhang W, Liu Q, Sun S, Tian Y, Li H, Huang J, Gu C, Liu S, Feng X
Cell death & disease 2020 May 5;11(5):319

Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.

Liu W, Fu R, Wang Z, Liu S, Tang C, Li L, Yin D
Journal of neuropathology and experimental neurology 2020 Jan 1;79(1):46-61

Latcripin-7A, derivative of Lentinula edodes C(91-3), reduces migration and induces apoptosis, autophagy, and cell cycle arrest at G(1) phase in breast cancer cells.

Din SRU, Zhong M, Nisar MA, Saleem MZ, Hussain A, Khinsar KH, Alam S, Ayub G, Kanwal S, Li X, Zhang W, Wang X, Ning A, Cao J, Huang M
Applied microbiology and biotechnology 2020 Dec;104(23):10165-10179

Proteomic Profile of Carbonylated Proteins Screen the Regulation of Calmodulin-Dependent Protein Kinases-AMPK-Beclin1 in Aerobic Exercise-Induced Autophagy in Middle-Aged Rat Hippocampus.

Liu W, Xia Y, Kuang H, Wang Z, Liu S, Tang C, Yin D
Gerontology 2019;65(6):620-633

Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling.

Guo Y, Pei X
Evidence-based complementary and alternative medicine : eCAM 2019;2019:7517431

Enhanced p62-NRF2 Feedback Loop due to Impaired Autophagic Flux Contributes to Arsenic-Induced Malignant Transformation of Human Keratinocytes.

Wu X, Sun R, Wang H, Yang B, Wang F, Xu H, Chen S, Zhao R, Pi J, Xu Y
Oxidative medicine and cellular longevity 2019;2019:1038932

EIF2A promotes cell survival during paclitaxel treatment in vitro and in vivo.

Chen L, He J, Zhou J, Xiao Z, Ding N, Duan Y, Li W, Sun LQ
Journal of cellular and molecular medicine 2019 Sep;23(9):6060-6071

The balance of apoptosis and autophagy via regulation of the AMPK signal pathway in aging rat striatum during regular aerobic exercise.

Liu W, Wang Z, Xia Y, Kuang H, Liu S, Li L, Tang C, Yin D
Experimental gerontology 2019 Sep;124:110647

Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears.

Zheng R, Huang S, Zhu J, Lin W, Xu H, Zheng X
Cell and tissue research 2019 Oct;378(1):113-125

Inhibition of high mobility group box 1 (HMGB1) attenuates podocyte apoptosis and epithelial-mesenchymal transition by regulating autophagy flux.

Jin J, Gong J, Zhao L, Zhang H, He Q, Jiang X
Journal of diabetes 2019 Oct;11(10):826-836

In Vivo Autophagy Up-Regulation of Small Intestine Enterocytes in Chinese Soft-Shelled Turtles during Hibernation.

Vistro WA, Zhang Y, Bai X, Yang P, Huang Y, Qu W, Baloch AS, Wu R, Tarique I, Chen Q
Biomolecules 2019 Nov 1;9(11)

α-hederin induces autophagic cell death in colorectal cancer cells through reactive oxygen species dependent AMPK/mTOR signaling pathway activation.

Sun J, Feng Y, Wang Y, Ji Q, Cai G, Shi L, Wang Y, Huang Y, Zhang J, Li Q
International journal of oncology 2019 May;54(5):1601-1612

Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte.

Jin J, Shi Y, Gong J, Zhao L, Li Y, He Q, Huang H
Stem cell research & therapy 2019 Mar 15;10(1):95

Mitochondrial dynamics modulation as a critical contribution for Shenmai injection in attenuating hypoxia/reoxygenation injury.

Yu J, Li Y, Liu X, Ma Z, Michael S, Orgah JO, Fan G, Zhu Y
Journal of ethnopharmacology 2019 Jun 12;237:9-19

LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle.

Tarique I, Vistro WA, Bai X, Yang P, Hong C, Huang Y, Haseeb A, Liu E, Gandahi NS, Xu M, Liu Y, Chen Q
Reproductive biology and endocrinology : RB&E 2019 Feb 9;17(1):19

Acteoside inhibits autophagic apoptosis of retinal ganglion cells to rescue glaucoma-induced optic atrophy.

Chen Q, Xi X, Zeng Y, He Z, Zhao J, Li Y
Journal of cellular biochemistry 2019 Aug;120(8):13133-13140

The Interrelationship of Pharmacologic Ascorbate Induced Cell Death and Ferroptosis.

Lőrincz T, Holczer M, Kapuy O, Szarka A
Pathology oncology research : POR 2019 Apr;25(2):669-679

Up-Regulation Thioredoxin Inhibits Advanced Glycation End Products-Induced Neurodegeneration.

Ren X, Wang NN, Qi H, Qiu YY, Zhang CH, Brown E, Kong H, Kong L
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;50(5):1673-1686

Rapamycin Reduces Podocyte Apoptosis and is Involved in Autophagy and mTOR/ P70S6K/4EBP1 Signaling.

Jin J, Hu K, Ye M, Wu D, He Q
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(2):765-772

Influence of outer membrane vesicles of Proteus mirabilis isolated from boar semen on sperm function.

Gao H, Gao Y, Yang C, Dong D, Yang J, Peng G, Peng J, Wang Y, Pan C, Dong W
Veterinary microbiology 2018 Oct;224:34-42

Tripterygium glycoside protects against puromycin amino nucleoside‑induced podocyte injury by upregulating autophagy.

Gong J, Jin J, Zhao L, Li Y, Li Y, He Q
International journal of molecular medicine 2018 Jul;42(1):115-122

SOCE induced calcium overload regulates autophagy in acute pancreatitis via calcineurin activation.

Zhu ZD, Yu T, Liu HJ, Jin J, He J
Cell death & disease 2018 Jan 19;9(2):50

Novel racemosin B derivatives as new therapeutic agents for aggressive breast cancer.

Xiao X, Xu M, Yang C, Yao Y, Liang LN, Ed Chung P, Long Q, Zacksenhaus E, He Z, Liu S, Ben-David Y
Bioorganic & medicinal chemistry 2018 Dec 15;26(23-24):6096-6104

Amino acid starvation-induced autophagy is involved in reduced subcutaneous fat deposition in weaning piglets derived from sows fed low-protein diet during gestation and lactation : Autophagy is involved in reduced fat deposition in maternal low-protein piglets.

Pan S, Jia Y, Yang X, Cai D, Liu Z, Song H, Zhao R
European journal of nutrition 2018 Apr;57(3):991-1001

Doxorubicin combined with low intensity ultrasound suppresses the growth of oral squamous cell carcinoma in culture and in xenografts.

Fan H, Li H, Liu G, Cong W, Zhao H, Cao W, Zheng J
Journal of experimental & clinical cancer research : CR 2017 Nov 21;36(1):163

Calcium release induced by 2-pyridinecarboxaldehyde thiosemicarbazone and its copper complex contributes to tumor cell death.

Fu Y, Liu Y, Wang J, Li C, Zhou S, Yang Y, Zhou P, Lu C, Li C
Oncology reports 2017 Mar;37(3):1662-1670

Berberine-induced autophagic cell death by elevating GRP78 levels in cancer cells.

La X, Zhang L, Li Z, Yang P, Wang Y
Oncotarget 2017 Mar 28;8(13):20909-20924

7-O-geranylquercetin-induced autophagy contributes to apoptosis via ROS generation in human non-small cell lung cancer cells.

Wang EX, Zou BY, Shi L, Du LY, Zhu YY, Jiang YM, Ma XD, Kang XH, Wang CY, Zhen YH, Sun LD
Life sciences 2017 Jul 1;180:102-113

A positive feedback loop between GRP78 and VPS34 is critical for GRP78-mediated autophagy in cancer cells.

Wang Y, Wu H, Li Z, Yang P, Li Z
Experimental cell research 2017 Feb 1;351(1):24-35

ABT737 induces mitochondrial pathway apoptosis and mitophagy by regulating DRP1-dependent mitochondrial fission in human ovarian cancer cells.

Yu Y, Xu L, Qi L, Wang C, Xu N, Liu S, Li S, Tian H, Liu W, Xu Y, Li Z
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Dec;96:22-29

Hydrogen sulfide decreases high glucose/palmitate-induced autophagy in endothelial cells by the Nrf2-ROS-AMPK signaling pathway.

Liu J, Wu J, Sun A, Sun Y, Yu X, Liu N, Dong S, Yang F, Zhang L, Zhong X, Xu C, Lu F, Zhang W
Cell & bioscience 2016;6:33

CLOCK Promotes Endothelial Damage by Inducing Autophagy through Reactive Oxygen Species.

Tang X, Lin C, Guo D, Qian R, Li X, Shi Z, Liu J, Li X, Fan L
Oxidative medicine and cellular longevity 2016;2016:9591482

Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF-κB signaling in murine breast cancer cells.

Xu Y, Wu D, Fan Y, Li P, Du H, Shi J, Wang D, Zhou X
Oncology letters 2016 Oct;12(4):2632-2640

AKT/TSC2/p70S6K signaling pathway is involved in quinocetone-induced death-promoting autophagy in HepG2 cells.

Zhang S, Zhang C, Tang S, Deng S, Zhou Y, Dai C, Yang X, Xiao X
Toxicology mechanisms and methods 2016 May;26(4):301-10

Excessive apoptosis and defective autophagy contribute to developmental testicular toxicity induced by fluoride.

Zhang S, Niu Q, Gao H, Ma R, Lei R, Zhang C, Xia T, Li P, Xu C, Wang C, Chen J, Dong L, Zhao Q, Wang A
Environmental pollution (Barking, Essex : 1987) 2016 May;212:97-104

Redox cycling of a copper complex with benzaldehyde nitrogen mustard-2-pyridine carboxylic acid hydrazone contributes to its enhanced antitumor activity, but no change in the mechanism of action occurs after chelation.

Yang Y, Li C, Fu Y, Liu Y, Zhang Y, Zhang Y, Zhou P, Yuan Y, Zhou S, Li S, Li C
Oncology reports 2016 Mar;35(3):1636-44

Promotion of Proapoptotic Signals by Lysosomal Photodamage: Mechanistic Aspects and Influence of Autophagy.

Kessel D, Evans CL
Photochemistry and photobiology 2016 Jul;92(4):620-3

Preventive Effects of a Novel Polysaccharide from Sepia esculenta Ink on Ovarian Failure and Its Action Mechanisms in Cyclophosphamide-Treated Mice.

Liu HZ, Tao YX, Luo P, Deng CM, Gu YP, Yang L, Zhong JP
Journal of agricultural and food chemistry 2016 Jul 20;64(28):5759-66

Overexpression of RBM5 induces autophagy in human lung adenocarcinoma cells.

Su Z, Wang K, Li R, Yin J, Hao Y, Lv X, Li J, Zhao L, Du Y, Li P, Zhang J
World journal of surgical oncology 2016 Feb 29;14:57

Early onset of cardiomyopathy and intellectual disability in a girl with Danon disease associated with a de novo novel mutation of the LAMP2 gene.

Sugie K, Yoshizawa H, Onoue K, Nakanishi Y, Eura N, Ogawa M, Nakano T, Sakaguchi Y, Hayashi YK, Kishimoto T, Shima M, Saito Y, Nishino I, Ueno S
Neuropathology : official journal of the Japanese Society of Neuropathology 2016 Dec;36(6):561-565

Copper Ion Attenuated the Antiproliferative Activity of Di-2-pyridylhydrazone Dithiocarbamate Derivative; However, There Was a Lack of Correlation between ROS Generation and Antiproliferative Activity.

Wang T, Fu Y, Huang T, Liu Y, Wu M, Yuan Y, Li S, Li C
Molecules (Basel, Switzerland) 2016 Aug 20;21(8)

Quinocetone triggered ER stress-induced autophagy via ATF6/DAPK1-modulated mAtg9a trafficking.

Zhou Y, Zhang S, Dai C, Tang S, Yang X, Li D, Zhao K, Xiao X
Cell biology and toxicology 2016 Apr;32(2):141-52

Identification and characterization of an endoplasmic reticulum localization motif.

Nie YW, Wang M, Zhang P, Huan L, Liu DJ
Biochemical and biophysical research communications 2016 Apr 22;473(1):249-254

Potent effects of dioscin against obesity in mice.

Liu M, Xu L, Yin L, Qi Y, Xu Y, Han X, Zhao Y, Sun H, Yao J, Lin Y, Liu K, Peng J
Scientific reports 2015 Jan 22;5:7973

Induction of autophagy improves embryo viability in cloned mouse embryos.

Shen X, Zhang N, Wang Z, Bai G, Zheng Z, Gu Y, Wu Y, Liu H, Zhou D, Lei L
Scientific reports 2015 Dec 8;5:17829

Thiopeptide Antibiotics Exhibit a Dual Mode of Action against Intracellular Pathogens by Affecting Both Host and Microbe.

Zheng Q, Wang Q, Wang S, Wu J, Gao Q, Liu W
Chemistry & biology 2015 Aug 20;22(8):1002-7

Unsaturated fatty acids induce non-canonical autophagy.

Niso-Santano M, Malik SA, Pietrocola F, Bravo-San Pedro JM, Mariño G, Cianfanelli V, Ben-Younès A, Troncoso R, Markaki M, Sica V, Izzo V, Chaba K, Bauvy C, Dupont N, Kepp O, Rockenfeller P, Wolinski H, Madeo F, Lavandero S, Codogno P, Harper F, Pierron G, Tavernarakis N, Cecconi F, Maiuri MC, Galluzzi L, Kroemer G
The EMBO journal 2015 Apr 15;34(8):1025-41

A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells.

Jin Y, Chen C, Meng L, Chen J, Li M, Zhu Z, Lin J
The Analyst 2012 Dec 7;137(23):5571-5

Autophagy is essential for mouse sense of balance.

Mariño G, Fernández AF, Cabrera S, Lundberg YW, Cabanillas R, Rodríguez F, Salvador-Montoliu N, Vega JA, Germanà A, Fueyo A, Freije JM, López-Otín C
The Journal of clinical investigation 2010 Jul;120(7):2331-44

Assessing autophagy in the context of photodynamic therapy.

Reiners JJ Jr, Agostinis P, Berg K, Oleinick NL, Kessel D
Autophagy 2010 Jan;6(1):7-18

Initiation of autophagy by photodynamic therapy.

Kessel D, Oleinick NL
Methods in enzymology 2009;453:1-16

Apoptotic and autophagic responses to Bcl-2 inhibition and photodamage.

Kessel D, Arroyo AS
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 2007 Dec;6(12):1290-5

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The LC3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human LC3. This antibody recognizes human,mouse,rat,zebrafish antigen. The LC3 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 12135-1-AP.pdf

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