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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- AM1800a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1800a, RRID:AB_2137696
- Product name
- LC3 Antibody (APG8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Purified Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.4 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references The effect of RNAi silencing of p62 using an osmotic polysorbitol transporter on autophagy and tumorigenesis in lungs of K-rasLA1 mice.
Induction of autophagy is essential for monocyte-macrophage differentiation.
The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5.
Characterization of Puma-dependent and Puma-independent neuronal cell death pathways following prolonged proteasomal inhibition.
The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
Islam MA, Shin JY, Yun CH, Cho CS, Seo HW, Chae C, Cho MH
Biomaterials 2014 Feb;35(5):1584-96
Biomaterials 2014 Feb;35(5):1584-96
Induction of autophagy is essential for monocyte-macrophage differentiation.
Zhang Y, Morgan MJ, Chen K, Choksi S, Liu ZG
Blood 2012 Mar 22;119(12):2895-905
Blood 2012 Mar 22;119(12):2895-905
The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5.
Rouschop KM, van den Beucken T, Dubois L, Niessen H, Bussink J, Savelkouls K, Keulers T, Mujcic H, Landuyt W, Voncken JW, Lambin P, van der Kogel AJ, Koritzinsky M, Wouters BG
The Journal of clinical investigation 2010 Jan;120(1):127-41
The Journal of clinical investigation 2010 Jan;120(1):127-41
Characterization of Puma-dependent and Puma-independent neuronal cell death pathways following prolonged proteasomal inhibition.
Tuffy LP, Concannon CG, D'Orsi B, King MA, Woods I, Huber HJ, Ward MW, Prehn JH
Molecular and cellular biology 2010 Dec;30(23):5484-501
Molecular and cellular biology 2010 Dec;30(23):5484-501
The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
Byun JY, Yoon CH, An S, Park IC, Kang CM, Kim MJ, Lee SJ
Carcinogenesis 2009 Nov;30(11):1880-8
Carcinogenesis 2009 Nov;30(11):1880-8
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of anti-LC3 Mab (Cat. #AM1800a) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LC3 Mab (Cat. #AM1800a) at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent image of U251 cells stained with AM1800a LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 ¼M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1800a LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells."
- Primary Ab dilution
- 1:200
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8)(Cat. #AM1800a). U251 cells(right) were treated with Chloroquine (50 ¼M,16h). AM1800a was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue)."
- Primary Ab dilution
- 1:25
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8) (Cat. #AM1800a). U251 cells(right) were treated with Chloroquine (50 ¼M,16h). AM1800a was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue)."
- Primary Ab dilution
- 1:25
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is Cat# AM1800a. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center."
- Primary Ab dilution
- 1:50~100
