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LearnPA5-35194
antibody from Invitrogen Antibodies
Targeting: MAP1LC3A
ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-35194 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LC3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAP1LC3B in mouse brain tissue lysate (35 µg/lane) using a MAP1LC3B polyclonal antibody (Product # PA5-35194).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAP1LC3B in rat brain lysate using a MAP1LC3B polyclonal antibody (Product # PA5-35194). Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MAP1LC3B showing staining in the cytoplasm of U-251 cells using a MAP1LC3B polyclonal antibody (Product # PA5-35194) followed by detection using a fluorescent conjugated secondary antibody (green) (1:1000, 1h). Nuclei were stained with Hoechst 33342 (blue) (10 µg/mL, 5 min).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MAP1LC3B showing staining in the cytoplasm of U-251 cells using a MAP1LC3B polyclonal antibody (Product # PA5-35194) followed by detection using a fluorescent conjugated secondary antibody (green) (1:1000, 1h). Nuclei were stained with Hoechst 33342 (blue) (10 µg/mL, 5 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MAP1LC3B in formalin-fixed, paraffin-embedded human brain tissue using a MAP1LC3B polyclonal antibody (Product # PA5-35194) followed by DAB staining.
