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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324624 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal LC3 Antibody (APG8A) (N-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal LC3 Antibody (APG8A) (N-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MAP1LC3A
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LC3 (APG8a) Pab (Cat. #TA324624) in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- APG8a (MAP1LC3A) Antibody (M1) (Cat. #TA324624) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the APG8a (MAP1LC3A) antibody detected the APG8a (MAP1LC3A) protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cells stained with TA324624 LC3 (APG8A) (N-term) antibody. U251 cells were treated with Chloroquine , then incubated with TA324624 LC3 (APG8A) (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
