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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- TA324626 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Cleaved LC3A Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Cleaved LC3A Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MAP1LC3A
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.35 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-cleaved-LC3 (APG8a) Pab (Cat. #TA324626) in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- 5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in 4% of paraformaldehyde. Treatment with Cat# TA324626 antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cells stained with cleaved LC3A antibody. U251 cells were treated with Chloroquine , then incubated with TA324626 cleaved LC3A primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cells stained with cleaved LC3A antibody. U251 cells were incubated with TA324626 cleaved LC3A primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Validation comment
- IHC
