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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG20462 - Provider product page
- Provider
- Arigo
- Product name
- anti-LC3A antibody [166AT1234]
- Antibody type
- Monoclonal
- Antigen
- A full length recombinant protein of human LC3A (NP_115903.1)
- Description
- Affinity purification with immunogen.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 166AT1234
- Vial size
- 50 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: 1) Y79 (soluble fraction of cell extract) and 2) 293 transfected with human LC3 (whole cell extract) stained with ARG20462 anti-LC3A antibody [166AT1234] at 8 µg/ml dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: HeLa cell lysates stained with ARG20462 anti-LC3A antibody [166AT1234], which were treated with rapamycin or bafilomycin overnight.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: (Left) untreated U251 (Right) Chloroquine (50 µM, 16h) treated U251 cells stained with ARG20462 anti-LC3A antibody [166AT1234] at 1:25 dilution followed by Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: 10X (lower panel) and 20X (upper panel) images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food stained with ARG20462 anti-LC3A antibody [166AT1234].
