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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- ARG54962 - Provider product page
- Provider
- Arigo
- Product name
- anti-LC3A (cleaved) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 89-120 from Human Cleaved LC3A.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: A431 cell line, untreated or treated with chloroquine, 100 ng/ml stained with ARG54962 anti-LC3A (cleaved) antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: mouse brain tissue lysate stained with ARG54962 anti-LC3A (cleaved) antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: 5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in 4% of paraformaldehyde. Staining with ARG54962 anti-LC3A (cleaved) antibody at 1:100 dilution. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cells stained with ARG54962 anti-LC3A (cleaved) antibody (green) at 1:100 dilution, 2 h at room temperature. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ARG54962 anti-LC3A (cleaved) antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cells stained with ARG54962 anti-LC3A (cleaved) antibody (green) at 1:100 dilution, 2 h at room temperature. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ARG54962 anti-LC3A (cleaved) antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded human cancer tissue stained with ARG54962 anti-LC3A (cleaved) antibody.
