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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- F46104 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- LC3A Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 30-56 from the human protein was used as the immunogen for this LC3A antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the LC3A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of LC3A antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LC3 gene (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of Chloroquine treated U251 cells (50 uM, 16h) stained with LC3A antibody. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE heart tissue stained with LC3A antibody.
