F46138

antibody from NSJ Bioreagents

Targeting: MAP1LC3A ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

F46138

Provider

NSJ Bioreagents

Product name

ATG8 Antibody

Antibody type

Polyclonal

Antigen

A portion of amino acids 89-120 from the human protein was used as the immunogen for this ATG8 antibody.

Description

Antigen affinity purified antibody

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the ATG8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of human A431 cells, untreated or treated with chloroquine (100ng/ml) tested with ATG8 antibody or beta-Actin Ab.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of mouse NIH3T3 cells, untreated or treated with chloroquine (100ng/ml) tested with ATG8 antibody or beta-Actin Ab.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent image of U251 cells stained with LC3A antibody (1:100). ATG8 immunoreactivity is localized to the autophagic vacuoles in the cytoplasm.