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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PAB0733 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0733, RRID:AB_1112896
- Product name
- MAP1LC3A polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAP1LC3A.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot on Peripheral Blood Mononuclear Cells (PBMCs) lysate using rabbit MAP1LC3A polyclonal antibody (Cat # PAB0733) at a dilution of 1 : 100 (ECL) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal microscopy on immunofluorescently detected MAP1LC3A in cytospin - isolated human white blood cells using rabbit MAP1LC3A polyclonal antibody (Cat # PAB0733) at a dilution of 1 : 200, incubated for 1 hr at room temperature. The cells were double - stained for MAP1LC3A appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour) .
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal microscopy on immunofluorescently detected MAP1LC3A in cytospin - isolated human white blood cells using rabbit MAP1LC3A polyclonal antibody (Cat # PAB0733) at a dilution of 1 : 200, incubated for 1 h at room temperature. The cells were double - stained for MHC class II appearing in green (A) and MAP1LC3A appearing in red (B) .
- Validation comment
- Immunocytochemistry
