A01543-1

antibody from Boster Biological Technology

Targeting: MAP1LC3A ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A01543-1

Provider

Boster Biological Technology

Product name

Anti-MAP1LC3A Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for MAP1LC3A detection. Tested with WB, IHC-P, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability.

Chen F, Yu X
Cardiovascular toxicology 2024 Jul;24(7):625-636

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Polyphyllin I Promotes Autophagic Cell Death and Apoptosis of Colon Cancer Cells via the ROS-Inhibited AKT/mTOR Pathway.

Luo Q, Jia L, Huang C, Qi Q, Jahangir A, Xia Y, Liu W, Shi R, Tang L, Chen Z
International journal of molecular sciences 2022 Aug 19;23(16)

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Association of astragaloside IV-inhibited autophagy and mineralization in vascular smooth muscle cells with lncRNA H19 and DUSP5-mediated ERK signaling.

Song Z, Wei D, Chen Y, Chen L, Bian Y, Shen Y, Chen J, Pan Y
Toxicology and applied pharmacology 2019 Feb 1;364:45-54

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-1). MAP1LC3A was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP1LC3A Antibody (A01543-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image