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LearnM01543-1
antibody from Boster Biological Technology
Targeting: MAP1LC3A
ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- M01543-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for LC3A/MAP1LC3A detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat LC3A/MAP1LC3A information: Molecular Weight: 14272 MW; Subcellular Localization: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome . LC3-II binds to the autophagic membranes; Tissue Specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- EOD-13
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability.
Ginsenoside Rb3 provides protective effects against cisplatin-induced nephrotoxicity via regulation of AMPK-/mTOR-mediated autophagy and inhibition of apoptosis in vitro and in vivo.
Endoplasmic reticulum stress triggers Xanthoangelol-induced protective autophagy via activation of JNK/c-Jun Axis in hepatocellular carcinoma.
Regulation of autophagy by AMP-activated protein kinase/sirtuin 1 pathway reduces spinal cord neurons damage.
Chen F, Yu X
Cardiovascular toxicology 2024 Jul;24(7):625-636
Cardiovascular toxicology 2024 Jul;24(7):625-636
Ginsenoside Rb3 provides protective effects against cisplatin-induced nephrotoxicity via regulation of AMPK-/mTOR-mediated autophagy and inhibition of apoptosis in vitro and in vivo.
Xing JJ, Hou JG, Ma ZN, Wang Z, Ren S, Wang YP, Liu WC, Chen C, Li W
Cell proliferation 2019 Jul;52(4):e12627
Cell proliferation 2019 Jul;52(4):e12627
Endoplasmic reticulum stress triggers Xanthoangelol-induced protective autophagy via activation of JNK/c-Jun Axis in hepatocellular carcinoma.
Li Z, Zhang L, Gao M, Han M, Liu K, Zhang Z, Gong Z, Xing L, Shi X, Lu K, Gao H
Journal of experimental & clinical cancer research : CR 2019 Jan 8;38(1):8
Journal of experimental & clinical cancer research : CR 2019 Jan 8;38(1):8
Regulation of autophagy by AMP-activated protein kinase/sirtuin 1 pathway reduces spinal cord neurons damage.
Yan P, Bai L, Lu W, Gao Y, Bi Y, Lv G
Iranian journal of basic medical sciences 2017 Sep;20(9):1029-1036
Iranian journal of basic medical sciences 2017 Sep;20(9):1029-1036
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human stomach, using MAP1LC3A Antibody(M01543-1)MAP1LC3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP1LC3A Antibody (M01543-1)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
