F46103-0.4ML

antibody from NSJ Bioreagents

Targeting: MAP1LC3A ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F46103-0.4ML

Provider

NSJ Bioreagents

Product name

LC3A Antibody / MAP1LC3A

Antibody type

Polyclonal

Description

Purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

0.4 ml, 0.08 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the LC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of LC3 antibody and rat brain lysate: Both lipidated (arrow, II) and non-lipidated (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

LC3 antibody western blot analysis in mouse brain tissue lysate

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent image of Chloroquine treated U251 cells (50 uM, 16h) stained with LC3 antibody. LC3 immunoreactivity is localized to autophagic vacuoles.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human brain tissue stained with LC3 antibody