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LearnAP21990PU-N
antibody from Acris Antibodies GmbH
Targeting: MAP1LC3A
ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AP21990PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21990PU-N, RRID:AB_10757288
- Product name
- anti LC3
- Antibody type
- Polyclonal
- Antigen
- Amino acids 1-50 of human MAP1 LC3A
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Zebrafish
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of LC3A in human brain lysate in the 1) absence and 2) presence of immunizing peptide, and in 3) mouse brain lysate, and 4) rat brain lysate using AP21990PU-N at 1.0 µg/ml, 1.0 µg/ml, 0.1 µg/ml and 0.1 µg/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MAP1 LC3A in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and AP21990PU-N (bottom left, right) at 5 µg/ml.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human brain tissue stained with MAP1LC3A antibody (Cat.-No.: AP21990PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
