Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 66139-1-Ig - Provider product page
- Provider
- Proteintech Group
- Product name
- LC3A/B antibody
- Antibody type
- Monoclonal
- Description
- LC3A/B antibody (Cat. #66139-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, pig, mouse and has been validated for the following applications: ELISA.
- Reactivity
- Human, Mouse, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 2B2B5
- Vial size
- 20ul, 150ul
Submitted references NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.
S-nitrosoglutathione reductase maintains mitochondrial homeostasis by promoting clearance of damaged mitochondria in porcine preimplantation embryos.
Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs.
AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.
cPKCγ-Modulated Sequential Reactivation of mTOR Inhibited Autophagic Flux in Neurons Exposed to Oxygen Glucose Deprivation/Reperfusion.
Pseudolaric acid B induced autophagy, but not apoptosis, in MRC5 human fibroblast cells.
The role of the chemokine receptor XCR1 in breast cancer cells.
Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.
Lithium alleviates neurotoxic prion peptide-induced synaptic damage and neuronal death partially by the upregulation of nuclear target REST and the restoration of Wnt signaling.
Autophagy is essential for flavopiridol‑induced cytotoxicity against MCF‑7 breast cancer cells.
Exogenous H2S facilitating ubiquitin aggregates clearance via autophagy attenuates type 2 diabetes-induced cardiomyopathy.
Colistin-Induced Apoptosis of Neuroblastoma-2a Cells Involves the Generation of Reactive Oxygen Species, Mitochondrial Dysfunction, and Autophagy.
Pseudolaric acid B activates autophagy in MCF-7 human breast cancer cells to prevent cell death.
Pseudolaric acid B inhibits proliferation in SW579 human thyroid squamous cell carcinoma.
Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells.
GRP78 is implicated in the modulation of tumor aerobic glycolysis by promoting autophagic degradation of IKKβ.
Suppression of autophagy augments the radiosensitizing effects of STAT3 inhibition on human glioma cells.
Rapamycin protects cardiomyocytes against anoxia/reoxygenation injury by inducing autophagy through the PI3k/Akt pathway.
SZC017, a novel oleanolic acid derivative, induces apoptosis and autophagy in human breast cancer cells.
Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer.
Gemcitabine resistance in breast cancer cells regulated by PI3K/AKT-mediated cellular proliferation exerts negative feedback via the MEK/MAPK and mTOR pathways.
Liu M, Zhang W, Li M, Feng J, Kuang W, Chen X, Yang F, Sun Q, Xu Z, Hua J, Yang C, Liu W, Shu Q, Yang Y, Zhou T, Xie S
Cell research 2021 Nov;31(11):1199-1211
Cell research 2021 Nov;31(11):1199-1211
S-nitrosoglutathione reductase maintains mitochondrial homeostasis by promoting clearance of damaged mitochondria in porcine preimplantation embryos.
Niu YJ, Zhou D, Cui XS
Cell proliferation 2021 Mar;54(3):e12990
Cell proliferation 2021 Mar;54(3):e12990
Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs.
Niu YJ, Zhou W, Nie ZW, Zhou D, Xu YN, Ock SA, Yan CG, Cui XS
Aging 2020 Jan 20;12(2):1256-1271
Aging 2020 Jan 20;12(2):1256-1271
AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.
Nie ZW, Niu YJ, Zhou W, Zhou DJ, Kim JY, Cui XS
Journal of cell science 2020 Dec 7;133(23)
Journal of cell science 2020 Dec 7;133(23)
cPKCγ-Modulated Sequential Reactivation of mTOR Inhibited Autophagic Flux in Neurons Exposed to Oxygen Glucose Deprivation/Reperfusion.
Hua R, Han S, Zhang N, Dai Q, Liu T, Li J
International journal of molecular sciences 2018 May 6;19(5)
International journal of molecular sciences 2018 May 6;19(5)
Pseudolaric acid B induced autophagy, but not apoptosis, in MRC5 human fibroblast cells.
Wang Y, Gao H, Wu T, Wang Z, Song F, Chen A, Zhang J, Zhang W, Zhang H, Yu J
Oncology letters 2018 Jan;15(1):863-870
Oncology letters 2018 Jan;15(1):863-870
The role of the chemokine receptor XCR1 in breast cancer cells.
Yang XL, Qi LG, Lin FJ, Ou ZL
Breast cancer (Dove Medical Press) 2017;9:227-236
Breast cancer (Dove Medical Press) 2017;9:227-236
Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.
Song Z, Shah SZA, Yang W, Dong H, Yang L, Zhou X, Zhao D
Frontiers in molecular neuroscience 2017;10:128
Frontiers in molecular neuroscience 2017;10:128
Lithium alleviates neurotoxic prion peptide-induced synaptic damage and neuronal death partially by the upregulation of nuclear target REST and the restoration of Wnt signaling.
Song Z, Yang W, Zhou X, Yang L, Zhao D
Neuropharmacology 2017 Sep 1;123:332-348
Neuropharmacology 2017 Sep 1;123:332-348
Autophagy is essential for flavopiridol‑induced cytotoxicity against MCF‑7 breast cancer cells.
Wang S, Wang K, Wang H, Han J, Sun H
Molecular medicine reports 2017 Dec;16(6):9715-9720
Molecular medicine reports 2017 Dec;16(6):9715-9720
Exogenous H2S facilitating ubiquitin aggregates clearance via autophagy attenuates type 2 diabetes-induced cardiomyopathy.
Wu J, Tian Z, Sun Y, Lu C, Liu N, Gao Z, Zhang L, Dong S, Yang F, Zhong X, Xu C, Lu F, Zhang W
Cell death & disease 2017 Aug 10;8(8):e2992
Cell death & disease 2017 Aug 10;8(8):e2992
Colistin-Induced Apoptosis of Neuroblastoma-2a Cells Involves the Generation of Reactive Oxygen Species, Mitochondrial Dysfunction, and Autophagy.
Dai C, Tang S, Velkov T, Xiao X
Molecular neurobiology 2016 Sep;53(7):4685-700
Molecular neurobiology 2016 Sep;53(7):4685-700
Pseudolaric acid B activates autophagy in MCF-7 human breast cancer cells to prevent cell death.
Yu J, Chen C, Xu T, Yan M, Xue B, Wang Y, Liu C, Zhong T, Wang Z, Meng X, Hu D, Yu X
Oncology letters 2016 Mar;11(3):1731-1737
Oncology letters 2016 Mar;11(3):1731-1737
Pseudolaric acid B inhibits proliferation in SW579 human thyroid squamous cell carcinoma.
Yu J, Ren P, Zhong T, Wang Y, Yan M, Xue B, Li R, Dai C, Liu C, Chen G, Yu XF
Molecular medicine reports 2015 Nov;12(5):7195-202
Molecular medicine reports 2015 Nov;12(5):7195-202
Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells.
Wu P, Zhu X, Jin W, Hao S, Liu Q, Zhang L
Biochemical and biophysical research communications 2015 May 1;460(2):183-90
Biochemical and biophysical research communications 2015 May 1;460(2):183-90
GRP78 is implicated in the modulation of tumor aerobic glycolysis by promoting autophagic degradation of IKKβ.
Li Z, Wang Y, Newton IP, Zhang L, Ji P, Li Z
Cellular signalling 2015 Jun;27(6):1237-45
Cellular signalling 2015 Jun;27(6):1237-45
Suppression of autophagy augments the radiosensitizing effects of STAT3 inhibition on human glioma cells.
Yuan X, Du J, Hua S, Zhang H, Gu C, Wang J, Yang L, Huang J, Yu J, Liu F
Experimental cell research 2015 Jan 15;330(2):267-276
Experimental cell research 2015 Jan 15;330(2):267-276
Rapamycin protects cardiomyocytes against anoxia/reoxygenation injury by inducing autophagy through the PI3k/Akt pathway.
Wang LQ, Cheng XS, Huang CH, Huang B, Liang Q
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2015 Feb;35(1):10-15
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2015 Feb;35(1):10-15
SZC017, a novel oleanolic acid derivative, induces apoptosis and autophagy in human breast cancer cells.
Gao L, Wang Y, Xu Z, Li X, Wu J, Liu S, Chu P, Sun Z, Sun B, Lin Y, Peng J, Han G, Wang S, Tang Z
Apoptosis : an international journal on programmed cell death 2015 Dec;20(12):1636-50
Apoptosis : an international journal on programmed cell death 2015 Dec;20(12):1636-50
Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer.
Sun Y, Liu Z, Zou X, Lan Y, Sun X, Wang X, Zhao S, Jiang C, Liu H
Journal of bioenergetics and biomembranes 2015 Aug;47(4):319-29
Journal of bioenergetics and biomembranes 2015 Aug;47(4):319-29
Gemcitabine resistance in breast cancer cells regulated by PI3K/AKT-mediated cellular proliferation exerts negative feedback via the MEK/MAPK and mTOR pathways.
Yang XL, Lin FJ, Guo YJ, Shao ZM, Ou ZL
OncoTargets and therapy 2014;7:1033-42
OncoTargets and therapy 2014;7:1033-42
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Western Blot analysis of MAP1LC3A/B in HeLa and HepG2 cells untreated/treated with 50?M chloroquine overnight using 66139-1-Ig (MAP1LC3A/B monoclonal antibody) at dilution of 1:1000.
- Sample type
- cell line
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 66139-1-Ig(LC3A/B antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The LC3A/B antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human LC3A/B. This antibody recognizes human antigen. The LC3A/B antibody has been validated for the following applications: ELISA, IHC, WB analysis.