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LearnABIN1449594
antibody from antibodies-online
Targeting: MAP1LC3A
ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- ABIN1449594 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 mg
Submitted references Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease.
Identification and anti-human glioblastoma activity of tagitinin C from Tithonia diversifolia methanolic extract.
IGF-I stimulates Rab7-RILP interaction during neuronal autophagy.
Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells.
Inhibition of isoprenylcysteine carboxylmethyltransferase induces autophagic-dependent apoptosis and impairs tumor growth.
Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent.
Immunohistochemical evidence for macroautophagy in neurones and endothelial cells in Alzheimer's disease.
The melanoma-associated transmembrane glycoprotein Gpnmb controls trafficking of cellular debris for degradation and is essential for tissue repair.
c-Abl and Arg tyrosine kinases regulate lysosomal degradation of the oncoprotein Galectin-3.
Dual inhibition of akt/mammalian target of rapamycin pathway by nanoparticle albumin-bound-rapamycin and perifosine induces antitumor activity in multiple myeloma.
Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression.
Autophagy protects neuron from Abeta-induced cytotoxicity.
Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection.
Superoxide is the major reactive oxygen species regulating autophagy.
Thermodynamic analysis of interfacial transport and of the thermomagnetoelectric system.
Nascimento-Ferreira I, Santos-Ferreira T, Sousa-Ferreira L, Auregan G, Onofre I, Alves S, Dufour N, Colomer Gould VF, Koeppen A, Déglon N, Pereira de Almeida L
Brain : a journal of neurology 2011 May;134(Pt 5):1400-15
Brain : a journal of neurology 2011 May;134(Pt 5):1400-15
Identification and anti-human glioblastoma activity of tagitinin C from Tithonia diversifolia methanolic extract.
Lee MY, Liao MH, Tsai YN, Chiu KH, Wen HC
Journal of agricultural and food chemistry 2011 Mar 23;59(6):2347-55
Journal of agricultural and food chemistry 2011 Mar 23;59(6):2347-55
IGF-I stimulates Rab7-RILP interaction during neuronal autophagy.
Bains M, Zaegel V, Mize-Berge J, Heidenreich KA
Neuroscience letters 2011 Jan 20;488(2):112-7
Neuroscience letters 2011 Jan 20;488(2):112-7
Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells.
Hino M, Hamada N, Tajika Y, Funayama T, Morimura Y, Sakashita T, Yokota Y, Fukamoto K, Mutou Y, Kobayashi Y, Yorifuji H
Journal of electron microscopy 2010;59(6):495-501
Journal of electron microscopy 2010;59(6):495-501
Inhibition of isoprenylcysteine carboxylmethyltransferase induces autophagic-dependent apoptosis and impairs tumor growth.
Wang M, Hossain MS, Tan W, Coolman B, Zhou J, Liu S, Casey PJ
Oncogene 2010 Sep 2;29(35):4959-70
Oncogene 2010 Sep 2;29(35):4959-70
Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent.
Geng Y, Kohli L, Klocke BJ, Roth KA
Neuro-oncology 2010 May;12(5):473-81
Neuro-oncology 2010 May;12(5):473-81
Immunohistochemical evidence for macroautophagy in neurones and endothelial cells in Alzheimer's disease.
Ma JF, Huang Y, Chen SD, Halliday G
Neuropathology and applied neurobiology 2010 Jun;36(4):312-9
Neuropathology and applied neurobiology 2010 Jun;36(4):312-9
The melanoma-associated transmembrane glycoprotein Gpnmb controls trafficking of cellular debris for degradation and is essential for tissue repair.
Li B, Castano AP, Hudson TE, Nowlin BT, Lin SL, Bonventre JV, Swanson KD, Duffield JS
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Dec;24(12):4767-81
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Dec;24(12):4767-81
c-Abl and Arg tyrosine kinases regulate lysosomal degradation of the oncoprotein Galectin-3.
Li X, Ma Q, Wang J, Liu X, Yang Y, Zhao H, Wang Y, Jin Y, Zeng J, Li J, Song L, Li X, Li P, Qian X, Cao C
Cell death and differentiation 2010 Aug;17(8):1277-87
Cell death and differentiation 2010 Aug;17(8):1277-87
Dual inhibition of akt/mammalian target of rapamycin pathway by nanoparticle albumin-bound-rapamycin and perifosine induces antitumor activity in multiple myeloma.
Cirstea D, Hideshima T, Rodig S, Santo L, Pozzi S, Vallet S, Ikeda H, Perrone G, Gorgun G, Patel K, Desai N, Sportelli P, Kapoor S, Vali S, Mukherjee S, Munshi NC, Anderson KC, Raje N
Molecular cancer therapeutics 2010 Apr;9(4):963-75
Molecular cancer therapeutics 2010 Apr;9(4):963-75
Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression.
Bohensky J, Leshinsky S, Srinivas V, Shapiro IM
Pediatric nephrology (Berlin, Germany) 2010 Apr;25(4):633-42
Pediatric nephrology (Berlin, Germany) 2010 Apr;25(4):633-42
Autophagy protects neuron from Abeta-induced cytotoxicity.
Hung SY, Huang WP, Liou HC, Fu WM
Autophagy 2009 May;5(4):502-10
Autophagy 2009 May;5(4):502-10
Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection.
English L, Chemali M, Duron J, Rondeau C, Laplante A, Gingras D, Alexander D, Leib D, Norbury C, Lippé R, Desjardins M
Nature immunology 2009 May;10(5):480-7
Nature immunology 2009 May;10(5):480-7
Superoxide is the major reactive oxygen species regulating autophagy.
Chen Y, Azad MB, Gibson SB
Cell death and differentiation 2009 Jul;16(7):1040-52
Cell death and differentiation 2009 Jul;16(7):1040-52
Thermodynamic analysis of interfacial transport and of the thermomagnetoelectric system.
Johnson M, Silsbee RH
Physical review. B, Condensed matter 1987 Apr 1;35(10):4959-4972
Physical review. B, Condensed matter 1987 Apr 1;35(10):4959-4972
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of LC3 Antibody Cat.-No AP32161PU-N in Rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis using LC3 Antibody Cat.-No AP32161PU-N in Mouse brain tissue lysates (35ug/lane).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Mouse brain cells using LC3 Antibody Cat.-No AP32161PU-N
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- LC3 and the CLN3-encoded protein, battenin, co-immunostaining. Wild-type and mutant cerebellar cells were costained with anti-LC3 Antibody Cat.-No AP32161PU-N (Red) and a monoclonal anti-Battenin Antibody(Green) and images were captured by Confocal Microscopy. Note the numerous larger vesicular structures, typical of mature AVs, stained with both the LC3 and battenin antibodies in wild-type cells (arrows), which are rare in mutant cells. Blue=DAPI. Protocol and data courtesy of Dr. Susan Cotman, Center for Human Genetic Research, Massachusetts General Hospital.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody Cat.-No AP32161PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
