Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-12402 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SRSF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, mouse, porcine and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references First Identification of RNA-Binding Proteins That Regulate Alternative Exons in the Dystrophin Gene.
Miro J, Bougé AL, Murauer E, Beyne E, Da Cunha D, Claustres M, Koenig M, Tuffery-Giraud S
International journal of molecular sciences 2020 Oct 21;21(20)
International journal of molecular sciences 2020 Oct 21;21(20)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a SFRS2 polyclonal antibody (Product # PA5-12402) in K562 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SRSF2 was achieved by transfecting HEK-293 with SRSF2 specific siRNAs (Silencer® select Product # s12730; s12728). Western blot analysis (Fig. a) was performed using whole cell extracts from the SRSF2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with SRSF2 Polyclonal Antibody (Product # PA5-12402, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SRSF2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) of HL-60 (Lane 1), SH-SY5Y (Lane 2), K-562 (Lane 3), PC-3 (Lane 4), SK-BR-3 (Lane 5), HEK-293 (Lane 6), U-2 OS (Lane 7), Jurkat (Lane 8) and Mouse Lung (Lane 9). The blot was probed with SRSF2 Polyclonal Antibody (Product # PA5-12402, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 32kDa band corresponding to SRSF2 was observed across the panel tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using a SFRS2 polyclonal antibody (Product # PA5-12402), followed by HRP-conjugated secondary antibody and DAB staining.