AP1810b

antibody from Abcepta

Targeting: ATG4C APG4C, AUTL1, AUTL3, FLJ14867

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1810b

Provider

Abcepta

Proper citation

Abgent Cat#AP1810b, RRID:AB_636736

Product name

ATG4C Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates.

Li M, Hou Y, Wang J, Chen X, Shao ZM, Yin XM
The Journal of biological chemistry 2011 Mar 4;286(9):7327-38

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of anti-hAPG4C-I182 Pab (Cat. #AP1810b) in 293 cell line lysates transiently transfected with the ATG4C gene (2ug/lane). hAPG4C-I182(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

The anti-APG4C Pab (Cat. #AP1810b) is used in Western blot to detect APG4C in Hela tissue lysate

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100