PA5-23141

antibody from Invitrogen Antibodies

Targeting: TAAR1 TA1, TAR1, TRAR1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-23141

Provider

Invitrogen Antibodies

Product name

TAAR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Thyronamine regulation of TAAR1 expression in breast cancer cells and investigation of its influence on viability and migration.

Tremmel E, Hofmann S, Kuhn C, Heidegger H, Heublein S, Hermelink K, Wuerstlein R, Harbeck N, Mayr D, Mahner S, Ditsch N, Jeschke U, Vattai A
Breast cancer (Dove Medical Press) 2019;11:87-97

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Western blot analysis of TAAR1 protein expression in MCF7 and T47D cells after stimulation with T 1 AM. Notes: Image of Western blot membranes with standard protein ladder (Page Ruler Prestained Protein ladder; # 26616; Thermo Fisher Scientific, Waltham, MA, USA); ( A , C , and E ) (left) Control membrane with b-actin. ( B , D , and F ) (right) Membrane incubated with TAAR1. Gray boxes mark the binding region of the TAAR1 antibody according to its molecular weight. ( A and B ) Nine samples of MCF7 cells: MCF7 cell control groups in columns 1), 4), and 7) and MCF7 cells stimulated with 1 nM T 1 AM in columns 2), 5), and 8) and stimulated with 10 nM T 1 AM in columns 3), 6), and 9). ( C and D ) Nine samples of T47D cells: T47D cell control groups in columns 1), 4), and 7) and T47D cells stimulated with 1 nM T 1 AM in columns 2), 5), and 8) and stimulated with 10 nM T 1 AM in columns 3), 6), and 9). ( E and F ) Six samples of T47D: T47D cell control groups in columns 1), 3), and 5) and cells stimulated with 10 nM T 1 AM and 10 ug/mL estradiol in columns 2), 4), and 6). Abbreviation: T 1 AM, 3-iodothyronamine.