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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28324 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RABGGTB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, Mouse brain, Rat brain. Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (91%), Xenopus laevis (91%), Pig (98%), Sheep (93%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RABGGTB using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a RABGGTB polyclonal antibody (Product # PA5-28324) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RABGGTB was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RABGGTB Polyclonal Antibody (Product # PA5-28324) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RABGGTB Polyclonal Antibody (Product # PA5-28324). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. RABGGTB Polyclonal Antibody (Product # PA5-28324) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RABGGTB Polyclonal Antibody (Product # PA5-28324). Sample (50 µg of whole cell lysate). Lane A: Rat brain. 10% SDS PAGE. RABGGTB Polyclonal Antibody (Product # PA5-28324) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RABGGTB Polyclonal Antibody detects RABGGTB protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RABGGTB protein stained by RABGGTB Polyclonal Antibody (Product # PA5-28324) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RABGGTB (Product # PA5-28324) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
